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OPENSEQ.org
PGK - Phosphoglycerate kinase
UniProt: P0A799 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10703
Length: 387 (383)
Sequences: 1848
Seq/Len: 4.83
PGK
Paralog alert: 0.04 [within 20: 0.01] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
186_V 301_A 4.247 1.00
228_D 279_A 3.848 1.00
374_V 379_A 3.802 1.00
17_F 57_T 3.49 1.00
34_D 38_R 3.46 1.00
289_A 292_Q 3.374 1.00
27_K 30_K 3.136 1.00
255_S 293_E 2.928 1.00
231_K 280_D 2.847 1.00
47_A 52_A 2.69 1.00
316_P 349_L 2.648 1.00
80_Y 83_D 2.57 1.00
17_F 154_I 2.53 1.00
273_S 289_A 2.516 1.00
168_A 172_D 2.505 1.00
18_I 47_A 2.453 1.00
45_E 49_K 2.438 1.00
46_L 165_P 2.41 1.00
323_E 327_N 2.299 1.00
260_A 267_A 2.27 1.00
125_L 129_Y 2.245 1.00
101_V 105_E 2.224 1.00
90_R 105_E 2.207 1.00
182_A 302_K 2.205 1.00
101_V 107_V 2.205 1.00
255_S 289_A 2.193 1.00
252_P 297_I 2.182 1.00
261_T 268_P 2.166 1.00
292_Q 296_E 2.166 1.00
219_N 283_I 2.161 1.00
53_K 103_E 2.152 1.00
22_L 40_S 2.076 1.00
223_A 236_A 2.073 1.00
95_Y 115_N 2.072 1.00
102_A 105_E 2.05 1.00
320_K 323_E 2.038 1.00
152_H 156_K 1.998 1.00
291_A 295_A 1.978 1.00
82_K 88_P 1.968 1.00
34_D 80_Y 1.953 1.00
57_T 133_C 1.925 1.00
329_I 336_S 1.924 1.00
46_L 50_Q 1.921 1.00
208_A 211_L 1.914 1.00
270_T 281_E 1.885 1.00
123_E 156_K 1.88 1.00
9_L 162_C 1.871 1.00
186_V 298_L 1.866 1.00
327_N 331_D 1.857 1.00
322_T 346_A 1.852 1.00
263_F 315_F 1.836 1.00
246_L 251_I 1.809 1.00
291_A 324_I 1.798 1.00
69_N 72_F 1.775 1.00
80_Y 84_K 1.763 1.00
323_E 350_F 1.758 1.00
202_D 244_R 1.752 1.00
214_G 310_V 1.737 1.00
171_L 378_V 1.693 0.99
143_T 151_T 1.693 0.99
177_A 337_I 1.658 0.99
11_L 47_A 1.655 0.99
261_T 281_E 1.644 0.99
155_G 380_M 1.635 0.99
7_T 165_P 1.629 0.99
206_K 248_T 1.618 0.99
202_D 248_T 1.593 0.99
260_A 284_L 1.586 0.99
75_L 79_N 1.582 0.99
73_S 91_L 1.582 0.99
290_S 294_L 1.577 0.99
271_L 288_D 1.563 0.99
239_V 243_K 1.561 0.99
223_A 239_V 1.56 0.99
163_A 381_L 1.558 0.99
94_D 98_G 1.547 0.99
295_A 299_K 1.526 0.99
305_L 370_V 1.516 0.99
127_K 157_F 1.512 0.98
87_N 105_E 1.511 0.98
198_L 238_L 1.511 0.98
127_K 131_A 1.51 0.98
261_T 280_D 1.509 0.98
186_V 210_Q 1.508 0.98
45_E 87_N 1.501 0.98
189_V 201_L 1.463 0.98
41_L 84_K 1.449 0.98
259_V 272_K 1.446 0.98
167_L 378_V 1.443 0.98
225_Q 246_L 1.432 0.98
12_A 103_E 1.429 0.98
15_R 55_M 1.428 0.98
316_P 319_R 1.421 0.97
238_L 241_E 1.419 0.97
250_N 297_I 1.417 0.97
273_S 276_D 1.414 0.97
240_D 243_K 1.413 0.97
57_T 112_V 1.397 0.97
44_I 56_V 1.391 0.97
299_K 331_D 1.387 0.97
13_G 103_E 1.376 0.97
277_V 283_I 1.368 0.97
99_V 107_V 1.36 0.96
115_N 128_K 1.359 0.96
48_L 53_K 1.347 0.96
265_E 317_N 1.338 0.96
7_T 46_L 1.333 0.96
55_M 133_C 1.332 0.96
195_S 235_E 1.315 0.96
20_A 56_V 1.314 0.95
17_F 133_C 1.311 0.95
112_V 125_L 1.297 0.95
257_V 274_V 1.296 0.95
210_Q 250_N 1.295 0.95
15_R 53_K 1.288 0.95
175_G 369_F 1.275 0.94
335_F 357_S 1.27 0.94
219_N 257_V 1.263 0.94
18_I 56_V 1.263 0.94
210_Q 297_I 1.262 0.94
291_A 320_K 1.256 0.94
6_M 47_A 1.254 0.94
212_I 294_L 1.253 0.94
147_A 152_H 1.25 0.94
293_E 297_I 1.246 0.94
18_I 43_T 1.243 0.93
348_D 353_A 1.236 0.93
295_A 328_A 1.235 0.93
210_Q 252_P 1.233 0.93
292_Q 295_A 1.226 0.93
38_R 84_K 1.226 0.93
92_V 107_V 1.219 0.93
266_T 315_F 1.215 0.92
296_E 299_K 1.209 0.92
173_A 335_F 1.209 0.92
58_S 143_T 1.208 0.92
7_T 168_A 1.207 0.92
269_A 317_N 1.204 0.92
210_Q 301_A 1.201 0.92
137_V 162_C 1.2 0.92
144_A 167_L 1.199 0.92
26_V 60_L 1.197 0.92
117_G 125_L 1.195 0.92
18_I 44_I 1.194 0.91
20_A 40_S 1.194 0.91
177_A 305_L 1.193 0.91
44_I 106_L 1.172 0.90
278_K 281_E 1.16 0.90
85_L 106_L 1.159 0.90
320_K 324_I 1.157 0.90
244_R 248_T 1.151 0.89
250_N 293_E 1.15 0.89
41_L 85_L 1.149 0.89
126_S 153_G 1.14 0.89
79_N 82_K 1.136 0.89
144_A 151_T 1.133 0.88
231_K 282_Q 1.133 0.88
296_E 300_N 1.124 0.88
212_I 290_S 1.122 0.88
115_N 125_L 1.12 0.88
78_V 82_K 1.119 0.88
174_L 369_F 1.118 0.87
27_K 265_E 1.116 0.87
88_P 105_E 1.114 0.87
244_R 247_T 1.112 0.87
344_L 348_D 1.111 0.87
243_K 247_T 1.109 0.87
64_T 67_E 1.103 0.87
82_K 108_V 1.097 0.86
96_L 115_N 1.092 0.86
298_L 325_V 1.092 0.86
261_T 267_A 1.085 0.85
67_E 116_K 1.085 0.85
261_T 278_K 1.081 0.85
210_Q 300_N 1.078 0.85
117_G 128_K 1.071 0.84
41_L 78_V 1.07 0.84
47_A 54_V 1.063 0.84
57_T 154_I 1.055 0.83
298_L 328_A 1.052 0.83
74_L 91_L 1.051 0.83
79_N 83_D 1.04 0.82
293_E 296_E 1.039 0.82
222_I 253_V 1.038 0.82
176_K 182_A 1.036 0.81
338_A 347_I 1.034 0.81
121_D 153_G 1.028 0.81
175_G 179_K 1.025 0.81
310_V 325_V 1.023 0.80
172_D 176_K 1.02 0.80
123_E 157_F 1.02 0.80
22_L 56_V 1.015 0.80
35_A 348_D 1.013 0.79
262_E 282_Q 1.012 0.79
96_L 125_L 1.011 0.79
265_E 316_P 1.008 0.79
75_L 93_K 1.007 0.79
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1phpA10.9845100-0.032Contact Map0.787
1zmrA11100-0.027Contact Map0.715
4feyA10.9974100-0.026Contact Map0.757
3q3vA20.9922100-0.023Contact Map0.79
1vpeA10.9871100-0.023Contact Map0.786
16pkA10.9845100-0.02Contact Map0.729
4dg5A10.9845100-0.018Contact Map0.738
1v6sA20.9716100-0.018Contact Map0.721
1qpgA10.9845100-0.017Contact Map0.694
3oz7A20.9793100-0.015Contact Map0.73
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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