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OPENSEQ.org
K6PF1 - 6-phosphofructokinase isozyme 1
UniProt: P0A796 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10699
Length: 320 (318)
Sequences: 2285
Seq/Len: 7.19
K6PF1
Paralog alert: 0.25 [within 20: 0.08] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
4_K 34_E 4.26 1.00
34_E 52_Q 3.52 1.00
6_G 38_I 3.511 1.00
100_V 106_S 3.137 1.00
182_A 190_V 3.055 1.00
39_Y 51_V 2.911 1.00
141_F 258_V 2.763 1.00
88_E 92_K 2.661 1.00
90_L 95_I 2.635 1.00
111_M 115_E 2.554 1.00
207_K 211_A 2.377 1.00
233_H 237_K 2.371 1.00
54_D 57_S 2.359 1.00
147_V 177_L 2.343 1.00
178_T 220_A 2.316 1.00
283_R 294_H 2.313 1.00
275_L 294_H 2.247 1.00
151_I 220_A 2.23 1.00
4_K 36_M 2.204 1.00
7_V 24_V 2.177 1.00
37_G 69_L 2.173 1.00
48_D 92_K 2.147 1.00
280_Y 283_R 2.146 1.00
89_N 92_K 2.138 1.00
37_G 53_L 2.129 1.00
36_M 52_Q 2.082 1.00
48_D 93_R 2.065 1.00
43_L 47_E 2.054 1.00
4_K 94_G 2.042 1.00
28_A 35_V 2.023 1.00
50_M 93_R 1.998 1.00
6_G 95_I 1.997 1.00
39_Y 49_R 1.959 1.00
48_D 89_N 1.956 1.00
145_S 258_V 1.953 1.00
24_V 99_V 1.941 1.00
79_D 82_I 1.939 1.00
166_V 231_L 1.912 1.00
20_A 123_L 1.9 1.00
141_F 145_S 1.849 1.00
200_E 238_E 1.839 1.00
234_F 237_K 1.758 1.00
61_M 68_F 1.736 1.00
83_R 116_M 1.727 1.00
75_P 78_R 1.69 1.00
144_L 177_L 1.672 1.00
271_Y 274_D 1.671 1.00
42_Y 109_G 1.658 1.00
271_Y 292_L 1.654 1.00
203_V 235_I 1.632 1.00
234_F 238_E 1.622 1.00
150_A 251_I 1.611 1.00
84_A 88_E 1.568 1.00
208_A 211_A 1.565 1.00
40_D 70_G 1.555 1.00
140_F 180_A 1.55 1.00
286_G 293_V 1.546 1.00
36_M 50_M 1.546 1.00
107_Y 122_G 1.543 1.00
40_D 49_R 1.54 1.00
27_S 274_D 1.539 1.00
280_Y 294_H 1.538 1.00
33_L 277_L 1.516 0.99
85_V 88_E 1.51 0.99
283_R 296_D 1.508 0.99
151_I 181_A 1.507 0.99
40_D 162_Q 1.498 0.99
274_D 278_A 1.487 0.99
24_V 269_G 1.487 0.99
147_V 181_A 1.482 0.99
192_V 308_F 1.481 0.99
51_V 57_S 1.466 0.99
151_I 185_G 1.452 0.99
169_V 220_A 1.45 0.99
189_F 202_L 1.447 0.99
275_L 283_R 1.438 0.99
112_R 115_E 1.421 0.99
266_S 319_L 1.421 0.99
88_E 91_K 1.413 0.99
31_E 277_L 1.407 0.99
236_E 242_E 1.401 0.99
10_S 106_S 1.371 0.99
99_V 276_L 1.37 0.99
115_E 298_I 1.37 0.99
2_I 97_A 1.367 0.99
195_V 310_G 1.359 0.98
169_V 178_T 1.358 0.98
36_M 93_R 1.346 0.98
111_M 298_I 1.337 0.98
235_I 239_T 1.328 0.98
20_A 138_I 1.327 0.98
80_E 84_A 1.317 0.98
38_I 50_M 1.313 0.98
8_L 45_L 1.3 0.98
29_L 55_R 1.299 0.98
154_L 167_V 1.288 0.98
22_R 266_S 1.286 0.98
8_L 100_V 1.283 0.98
200_E 204_N 1.272 0.97
271_Y 275_L 1.264 0.97
138_I 265_A 1.262 0.97
38_I 45_L 1.262 0.97
152_D 262_R 1.259 0.97
144_L 147_V 1.253 0.97
100_V 110_A 1.25 0.97
288_Q 293_V 1.246 0.97
105_G 109_G 1.199 0.96
286_G 295_H 1.197 0.96
311_D 314_D 1.191 0.96
149_E 153_R 1.185 0.96
264_L 267_R 1.177 0.95
110_A 120_C 1.157 0.95
206_I 217_A 1.147 0.94
8_L 38_I 1.14 0.94
204_N 207_K 1.14 0.94
230_E 234_F 1.132 0.94
275_L 285_V 1.131 0.94
151_I 167_V 1.129 0.94
36_M 95_I 1.129 0.94
143_A 177_L 1.124 0.94
148_V 185_G 1.123 0.94
24_V 273_I 1.113 0.93
25_V 58_V 1.113 0.93
166_V 219_V 1.102 0.93
169_V 177_L 1.101 0.93
5_I 99_V 1.099 0.93
83_R 112_R 1.098 0.93
221_I 231_L 1.091 0.92
97_A 276_L 1.084 0.92
314_D 318_K 1.083 0.92
73_R 109_G 1.08 0.92
127_I 170_M 1.075 0.92
309_K 312_W 1.071 0.91
12_G 128_D 1.071 0.91
7_V 25_V 1.068 0.91
240_G 243_T 1.064 0.91
5_I 97_A 1.061 0.91
9_T 17_M 1.055 0.91
114_T 282_G 1.051 0.90
175_G 194_E 1.049 0.90
246_T 251_I 1.038 0.90
87_I 116_M 1.037 0.90
284_C 295_H 1.037 0.90
199_R 234_F 1.036 0.89
271_Y 294_H 1.032 0.89
84_A 116_M 1.03 0.89
46_Y 74_F 1.02 0.88
210_I 241_R 1.017 0.88
157_T 161_H 1.017 0.88
3_K 96_D 1.016 0.88
233_H 236_E 1.014 0.88
74_F 105_G 1.014 0.88
70_G 162_Q 1.012 0.88
121_I 294_H 1.012 0.88
175_G 193_P 1.003 0.87
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1pfkA21100-0.046Contact Map0.855
1zxxA10.9938100-0.042Contact Map0.773
4i7eA40.9938100-0.041Contact Map0.879
3o8lA20.99691000.002Contact Map0.782
3o8oB40.99691000.011Contact Map0.729
3opyB40.99691000.019Contact Map0.751
3opyA40.99381000.022Contact Map0.725
3o8oA40.99381000.024Contact Map0.763
3hnoA40.99381000.026Contact Map0.778
2higA20.97811000.042Contact Map0.796
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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