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OPENSEQ.org

MSCL - Large-conductance mechanosensitive channel
UniProt: P0A742 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11180
Length: 136 (133)
Sequences: 1246
Seq/Len: 9.37

MSCL
Paralog alert: 0.02 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
5_K 9_E 3.415 1.00
34_S 38_A 3.392 1.00
6_E 97_K 2.552 1.00
19_L 23_V 2.451 1.00
31_K 34_S 2.263 1.00
6_E 100_N 2.221 1.00
117_K 123_T 2.172 1.00
35_S 39_D 2.147 1.00
117_K 120_V 2.125 1.00
17_V 99_I 2.123 1.00
90_F 94_M 2.105 1.00
36_L 40_I 2.069 1.00
40_I 73_M 1.993 1.00
74_H 77_V 1.973 1.00
57_F 75_Y 1.797 1.00
65_Q 68_I 1.774 1.00
19_L 22_G 1.759 1.00
30_G 34_S 1.685 1.00
6_E 93_F 1.594 1.00
124_E 130_K 1.561 1.00
36_L 78_F 1.549 1.00
116_T 119_E 1.536 1.00
58_A 72_V 1.535 1.00
80_Q 84_D 1.491 1.00
31_K 84_D 1.363 0.99
114_A 120_V 1.362 0.99
119_E 123_T 1.359 0.99
39_D 77_V 1.349 0.99
66_G 69_P 1.345 0.99
11_A 89_A 1.309 0.99
3_I 90_F 1.296 0.99
65_Q 69_P 1.278 0.99
124_E 127_D 1.27 0.99
84_D 88_V 1.268 0.98
23_V 30_G 1.268 0.98
93_F 97_K 1.265 0.98
62_R 71_V 1.257 0.98
9_E 104_R 1.25 0.98
9_E 100_N 1.246 0.98
54_F 79_I 1.245 0.98
3_I 94_M 1.244 0.98
34_S 84_D 1.238 0.98
123_T 128_L 1.226 0.98
35_S 78_F 1.225 0.98
11_A 86_L 1.218 0.98
16_V 36_L 1.197 0.98
2_S 5_K 1.195 0.98
32_I 82_V 1.177 0.97
75_Y 79_I 1.176 0.97
17_V 95_A 1.164 0.97
34_S 37_V 1.103 0.96
115_P 118_E 1.098 0.95
18_D 21_V 1.097 0.95
39_D 81_N 1.085 0.95
22_G 100_N 1.084 0.95
68_I 71_V 1.075 0.95
42_M 46_G 1.064 0.94
80_Q 88_V 1.06 0.94
121_L 126_R 1.044 0.94
12_M 16_V 1.005 0.92
128_L 131_E 1.001 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2oarA50.94851000.107Contact Map0.51
3hzqA10.69851000.276Contact Map0.298
4lkuA50.213296.60.856Contact Map0.677
2kluA10.419117.20.94Contact Map0.447
2yfvC10.183812.20.944Contact Map0.234
1b9uA10.242610.50.946Contact Map0.064
2zy9A20.66916.50.951Contact Map0.165
3lw5I10.22063.70.956Contact Map0.124
4h33A10.84563.30.957Contact Map0.136
2nwlA30.90442.80.959Contact Map0.149

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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