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HYBF - Probable hydrogenase nickel incorporation protein HybF
UniProt: P0A703 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11804
Length: 113 (113)
Sequences: 782
Seq/Len: 6.92

HYBF
Paralog alert: 0.48 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: HYBF HYPA
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
48_F 62_L 2.961 1.00
48_F 60_C 2.643 1.00
75_D 96_R 2.522 1.00
23_V 112_V 2.455 1.00
32_E 106_I 2.345 1.00
28_A 63_H 2.196 1.00
32_E 65_V 2.101 1.00
28_A 61_D 2.071 1.00
72_W 98_R 1.977 1.00
72_W 79_V 1.974 1.00
17_Q 20_Q 1.931 1.00
32_E 108_K 1.92 1.00
72_W 77_S 1.879 1.00
14_I 26_V 1.872 1.00
27_T 113_E 1.811 1.00
54_G 58_Q 1.676 1.00
52_C 59_G 1.664 1.00
6_L 39_V 1.654 1.00
18_A 56_V 1.619 1.00
35_A 66_Y 1.6 1.00
14_I 110_I 1.598 1.00
71_A 80_V 1.568 1.00
78_Q 91_L 1.549 0.99
7_C 44_V 1.544 0.99
70_Q 101_T 1.532 0.99
9_S 13_I 1.528 0.99
30_W 109_S 1.498 0.99
43_A 46_F 1.464 0.99
70_Q 79_V 1.455 0.99
18_A 23_V 1.435 0.99
74_W 98_R 1.435 0.99
55_T 58_Q 1.432 0.99
35_A 68_P 1.41 0.99
71_A 97_L 1.398 0.99
6_L 38_C 1.381 0.99
107_V 110_I 1.34 0.98
14_I 107_V 1.29 0.97
30_W 63_H 1.264 0.97
31_L 64_I 1.245 0.97
80_V 97_L 1.236 0.97
34_G 102_G 1.209 0.96
53_H 58_Q 1.191 0.95
41_E 66_Y 1.191 0.95
67_K 104_S 1.175 0.95
25_R 59_G 1.165 0.95
11_V 57_A 1.165 0.95
28_A 111_E 1.15 0.94
7_C 43_A 1.107 0.93
5_S 9_S 1.089 0.92
12_E 15_Q 1.088 0.92
11_V 51_V 1.086 0.92
79_V 100_D 1.082 0.91
8_Q 51_V 1.08 0.91
14_I 29_V 1.079 0.91
43_A 47_S 1.054 0.90
42_S 45_R 1.037 0.89
13_I 107_V 1.027 0.88
7_C 10_A 1.012 0.87
74_W 96_R 1.004 0.87
10_A 31_L 1.001 0.86
18_A 26_V 1.001 0.86
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3a43A211000.042Contact Map0.805
2kdxA111000.062Contact Map0.724
2k2dA10.6549990.668Contact Map0.695
3pwfA20.831995.70.823Contact Map0.511
1lkoA10.88595.50.826Contact Map0.5
2lcqA10.911595.20.829Contact Map0.363
4aybP10.380594.80.833Contact Map0.042
1yuzA20.796594.50.836Contact Map0.593
1s24A10.5575830.871Contact Map0.751
3h0gL20.539881.70.873Contact Map0.535

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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