OPENSEQ.org - HSCB - ECOLI GREMLIN

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HSCB - Co-chaperone protein HscB

UniProt:	P0A6L9	Sequence MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQLAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREELDEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSSAEQLEEKLLDF	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG12131
Length:	171 (170)
Sequences:	554
Seq/Len:	3.26

HSCB
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
101_E	120_R	2.48	1.00
99_R	160_S	2.364	1.00
2_D	11_A	2.349	1.00
14_Q	133_V	2.29	1.00
3_Y	70_Y	2.177	1.00
114_L	161_A	2.073	1.00
135_Q	147_T	1.948	1.00
68_A	148_V	1.875	0.99
40_S	43_E	1.751	0.99
28_Q	151_L	1.741	0.99
26_D	29_R	1.731	0.99
93_M	100_E	1.709	0.99
95_Q	153_F	1.691	0.99
2_D	5_T	1.68	0.98
44_Q	51_S	1.622	0.98
24_F	60_T	1.583	0.98
140_T	143_A	1.555	0.97
60_T	70_Y	1.543	0.97
63_H	66_M	1.513	0.97
27_L	54_I	1.484	0.96
31_Y	54_I	1.482	0.96
23_R	27_L	1.48	0.96
134_E	138_N	1.455	0.95
6_L	42_A	1.454	0.95
12_R	137_D	1.418	0.94
64_P	93_M	1.414	0.94
44_Q	125_F	1.412	0.94
95_Q	98_L	1.366	0.93
88_D	165_E	1.353	0.92
2_D	35_K	1.341	0.92
3_Y	53_T	1.307	0.91
63_H	155_D	1.269	0.89
105_I	117_F	1.263	0.89
132_M	136_L	1.244	0.88
111_E	169_L	1.24	0.87
11_A	74_L	1.208	0.85
118_I	161_A	1.208	0.85
25_Q	152_R	1.206	0.85
48_V	52_A	1.204	0.85
50_Q	53_T	1.199	0.85
125_F	151_L	1.196	0.85
156_K	160_S	1.183	0.84
14_Q	130_Q	1.166	0.83
90_A	126_D	1.159	0.82
117_F	164_L	1.155	0.82
93_M	97_E	1.151	0.82
149_R	153_F	1.143	0.81
59_Q	66_M	1.136	0.80
25_Q	29_R	1.132	0.80
124_M	154_L	1.124	0.79
128_R	147_T	1.117	0.79
91_F	98_L	1.112	0.78
52_A	88_D	1.11	0.78
39_G	43_E	1.11	0.78
91_F	153_F	1.109	0.78
40_S	96_L	1.108	0.78
132_M	151_L	1.102	0.77
72_L	157_L	1.094	0.77
16_D	19_A	1.093	0.77
23_R	29_R	1.089	0.76
136_L	141_W	1.081	0.76
131_L	134_E	1.08	0.75
6_L	25_Q	1.061	0.74
41_Q	44_Q	1.061	0.74
69_E	151_L	1.061	0.74
8_G	44_Q	1.055	0.73
49_Q	54_I	1.054	0.73
45_L	95_Q	1.053	0.73
24_F	72_L	1.048	0.72
32_H	55_N	1.042	0.72
23_R	44_Q	1.04	0.71
91_F	99_R	1.038	0.71
132_M	135_Q	1.032	0.71
123_K	126_D	1.032	0.71
94_E	120_R	1.032	0.71
135_Q	144_A	1.03	0.70
126_D	130_Q	1.027	0.70
68_A	99_R	1.026	0.70
58_W	65_L	1.022	0.70
85_T	149_R	1.019	0.69
53_T	56_Q	1.018	0.69
28_Q	34_D	1.017	0.69
69_E	152_R	1.003	0.67
99_R	156_K	1.003	0.67

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
1fpoA	3	1	100	0.227	Contact Map	0.589
3hhoA	4	0.9942	100	0.227	Contact Map	0.569
3bvoA	2	0.9649	100	0.255	Contact Map	0.519
3uo3A	2	0.9298	100	0.32	Contact Map	0.534
2l6lA	1	0.8012	99.7	0.639	Contact Map	0.188
1wjzA	1	0.4561	99.4	0.716	Contact Map	0.486
1n4cA	1	0.386	99.3	0.729	Contact Map	0.239
1fafA	1	0.4035	99.3	0.73	Contact Map	0.216
2yuaA	1	0.4854	99.3	0.735	Contact Map	0.366
2cugA	1	0.4211	99.2	0.74	Contact Map	0.455

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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