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OPENSEQ.org
GLPK - Glycerol kinase
UniProt: P0A6F3 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10398
Length: 502 (494)
Sequences: 5502
Seq/Len: 11.14
GLPK
Paralog alert: 0.75 [within 20: 0.17] - ratio of genomes with paralogs
Cluster includes: ARAB FUCK GLPK LSRK LYXK RHAB XYLB YGCE
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
202_D 212_R 4.843 1.00
23_D 27_N 4.436 1.00
112_I 147_D 3.944 1.00
115_H 147_D 3.161 1.00
390_R 394_E 3.151 1.00
194_N 201_D 3.137 1.00
365_I 377_I 2.971 1.00
19_A 60_T 2.931 1.00
390_R 484_Y 2.912 1.00
220_R 223_E 2.831 1.00
421_Q 425_D 2.805 1.00
98_I 152_S 2.771 1.00
199_D 212_R 2.756 1.00
21_V 64_V 2.754 1.00
193_F 218_V 2.679 1.00
194_N 197_T 2.616 1.00
51_M 173_K 2.592 1.00
397_Q 429_T 2.59 1.00
457_N 460_E 2.569 1.00
91_E 155_R 2.552 1.00
456_Q 460_E 2.55 1.00
430_R 472_R 2.52 1.00
337_V 376_H 2.52 1.00
39_I 100_N 2.424 1.00
128_N 201_D 2.423 1.00
99_Y 149_V 2.419 1.00
10_L 57_Q 2.406 1.00
390_R 481_N 2.4 1.00
313_S 381_T 2.375 1.00
205_L 212_R 2.352 1.00
34_R 63_E 2.316 1.00
32_S 63_E 2.287 1.00
93_E 160_E 2.285 1.00
171_I 243_I 2.275 1.00
54_W 173_K 2.269 1.00
175_T 240_I 2.265 1.00
417_N 469_R 2.248 1.00
88_I 166_V 2.248 1.00
34_R 59_S 2.172 1.00
92_K 157_R 2.165 1.00
51_M 96_K 2.164 1.00
16_S 33_Q 2.153 1.00
244_A 249_A 2.148 1.00
270_C 310_A 2.146 1.00
202_D 206_E 2.14 1.00
20_V 441_A 2.107 1.00
336_K 375_N 2.025 1.00
397_Q 403_R 2.022 1.00
37_E 52_E 1.997 1.00
155_R 160_E 1.997 1.00
8_V 19_A 1.995 1.00
345_V 365_I 1.969 1.00
179_V 217_E 1.925 1.00
406_A 432_E 1.915 1.00
241_S 451_A 1.913 1.00
224_V 241_S 1.882 1.00
474_G 477_T 1.875 1.00
12_Q 17_S 1.87 1.00
341_N 376_H 1.83 1.00
92_K 162_L 1.828 1.00
128_N 203_K 1.798 1.00
418_F 422_F 1.749 1.00
329_D 332_Y 1.734 1.00
379_R 383_E 1.722 1.00
229_N 232_G 1.718 1.00
6_Y 29_I 1.708 1.00
344_Y 486_G 1.698 1.00
42_K 45_W 1.698 1.00
278_E 298_E 1.686 1.00
63_E 67_K 1.667 1.00
484_Y 488_K 1.649 1.00
417_N 471_F 1.649 1.00
114_E 118_R 1.645 1.00
143_K 147_D 1.625 1.00
409_V 420_M 1.624 1.00
95_G 172_W 1.623 1.00
8_V 61_L 1.607 1.00
373_N 376_H 1.604 1.00
358_D 495_M 1.586 1.00
89_V 214_M 1.584 1.00
21_V 70_I 1.584 1.00
124_Y 127_S 1.583 1.00
435_E 466_V 1.559 1.00
77_A 448_A 1.553 1.00
331_E 416_N 1.544 1.00
124_Y 128_N 1.543 1.00
7_I 449_G 1.541 1.00
196_H 280_A 1.535 1.00
219_R 223_E 1.534 1.00
124_Y 203_K 1.526 1.00
111_E 115_H 1.526 1.00
175_T 226_G 1.52 1.00
326_D 329_D 1.519 1.00
368_L 372_V 1.508 1.00
345_V 349_F 1.482 1.00
317_L 381_T 1.481 1.00
417_N 433_R 1.48 1.00
394_E 397_Q 1.48 1.00
171_I 181_V 1.478 1.00
115_H 119_D 1.466 1.00
224_V 227_Q 1.465 1.00
261_M 406_A 1.463 1.00
430_R 470_E 1.452 1.00
84_R 245_G 1.441 1.00
432_E 470_E 1.441 1.00
425_D 474_G 1.437 1.00
345_V 381_T 1.433 1.00
49_D 52_E 1.424 1.00
169_W 173_K 1.422 1.00
54_W 58_S 1.417 1.00
492_K 495_M 1.407 0.99
387_Y 483_R 1.404 0.99
151_G 154_E 1.398 0.99
89_V 145_I 1.391 0.99
144_W 148_H 1.389 0.99
30_S 64_V 1.385 0.99
330_S 378_I 1.385 0.99
262_A 404_L 1.38 0.99
32_S 60_T 1.377 0.99
6_Y 21_V 1.37 0.99
86_T 101_A 1.365 0.99
264_N 423_Q 1.354 0.99
181_V 219_R 1.353 0.99
244_A 447_L 1.351 0.99
325_N 329_D 1.348 0.99
343_V 372_V 1.344 0.99
433_R 471_F 1.342 0.99
363_G 368_L 1.339 0.99
17_S 57_Q 1.337 0.99
21_V 68_A 1.335 0.99
64_V 68_A 1.325 0.99
362_R 369_T 1.324 0.99
261_M 408_R 1.323 0.99
315_Q 319_D 1.322 0.99
17_S 56_T 1.322 0.99
112_I 143_K 1.309 0.99
422_F 426_I 1.307 0.99
386_A 422_F 1.303 0.99
307_V 388_Q 1.302 0.99
10_L 19_A 1.294 0.99
476_E 479_E 1.288 0.99
435_E 468_E 1.288 0.99
309_M 312_A 1.287 0.99
32_S 64_V 1.279 0.99
334_A 418_F 1.267 0.99
332_Y 336_K 1.266 0.99
224_V 239_P 1.257 0.99
146_L 153_R 1.255 0.99
5_K 76_A 1.254 0.99
479_E 482_Y 1.246 0.98
266_Y 423_Q 1.246 0.98
10_L 61_L 1.244 0.98
7_I 454_F 1.244 0.98
333_F 375_N 1.241 0.98
51_M 55_A 1.24 0.98
193_F 197_T 1.235 0.98
406_A 430_R 1.228 0.98
333_F 336_K 1.226 0.98
158_R 180_H 1.224 0.98
205_L 215_L 1.223 0.98
449_G 455_W 1.219 0.98
184_Y 198_L 1.218 0.98
329_D 333_F 1.21 0.98
219_R 225_Y 1.205 0.98
394_E 488_K 1.204 0.98
425_D 477_T 1.192 0.98
39_I 52_E 1.192 0.98
91_E 96_K 1.187 0.98
127_S 282_K 1.183 0.98
30_S 68_A 1.182 0.98
428_G 478_T 1.181 0.98
489_K 493_R 1.178 0.98
55_A 59_S 1.175 0.98
493_R 497_W 1.173 0.98
331_E 335_T 1.172 0.98
110_A 114_E 1.167 0.97
123_D 127_S 1.166 0.97
93_E 96_K 1.166 0.97
203_K 206_E 1.162 0.97
65_L 75_I 1.162 0.97
152_S 155_R 1.157 0.97
24_H 454_F 1.15 0.97
151_G 155_R 1.148 0.97
22_M 28_I 1.148 0.97
108_R 144_W 1.146 0.97
89_V 142_V 1.142 0.97
233_K 236_T 1.141 0.97
21_V 75_I 1.141 0.97
117_K 122_E 1.14 0.97
124_Y 207_V 1.138 0.97
50_P 100_N 1.137 0.97
390_R 427_L 1.134 0.97
78_I 238_I 1.132 0.97
53_I 166_V 1.131 0.97
211_P 214_M 1.13 0.97
313_S 345_V 1.123 0.97
415_A 437_R 1.118 0.96
185_T 293_C 1.116 0.96
420_M 471_F 1.114 0.96
113_C 133_I 1.11 0.96
8_V 21_V 1.109 0.96
224_V 451_A 1.105 0.96
46_V 105_Q 1.104 0.96
6_Y 74_Q 1.104 0.96
139_G 208_L 1.102 0.96
171_I 225_Y 1.102 0.96
49_D 100_N 1.099 0.96
386_A 423_Q 1.095 0.96
333_F 378_I 1.094 0.96
57_Q 170_L 1.092 0.96
91_E 160_E 1.091 0.96
125_I 191_M 1.091 0.96
62_V 66_A 1.091 0.96
258_K 405_H 1.091 0.96
228_T 238_I 1.089 0.96
364_A 494_A 1.084 0.96
121_L 207_V 1.084 0.96
36_F 56_T 1.083 0.96
85_E 189_R 1.081 0.95
184_Y 193_F 1.073 0.95
111_E 114_E 1.073 0.95
59_S 63_E 1.073 0.95
70_I 74_Q 1.07 0.95
365_I 381_T 1.065 0.95
199_D 217_E 1.059 0.95
153_R 156_A 1.059 0.95
385_I 413_A 1.058 0.95
394_E 484_Y 1.057 0.95
467_I 470_E 1.052 0.95
309_M 313_S 1.051 0.94
65_L 68_A 1.049 0.94
227_Q 237_R 1.047 0.94
342_G 379_R 1.043 0.94
387_Y 426_I 1.042 0.94
386_A 426_I 1.042 0.94
393_L 427_L 1.038 0.94
152_S 161_L 1.038 0.94
156_A 214_M 1.036 0.94
57_Q 61_L 1.028 0.94
379_R 418_F 1.028 0.94
80_I 170_L 1.026 0.94
227_Q 239_P 1.026 0.94
99_Y 145_I 1.025 0.93
107_R 134_D 1.024 0.93
38_Q 48_H 1.022 0.93
5_K 74_Q 1.022 0.93
77_A 241_S 1.022 0.93
149_V 152_S 1.019 0.93
428_G 480_R 1.018 0.93
196_H 279_K 1.014 0.93
247_Q 271_F 1.014 0.93
184_Y 218_V 1.009 0.93
50_P 88_I 1.005 0.93
321_M 377_I 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3ifrA20.97411000.109Contact Map0.737
3h3nX211000.112Contact Map0.807
3hz6A10.97211000.114Contact Map0.64
3g25A40.9921000.116Contact Map0.878
2p3rA80.9941000.121Contact Map0.883
3ll3A20.96811000.122Contact Map0.765
2zf5O20.98011000.124Contact Map0.849
2d4wA20.9921000.124Contact Map0.808
4e1jA40.98611000.132Contact Map0.819
2dpnA20.98611000.132Contact Map0.805
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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