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OPENSEQ.org
LEUC - 3-isopropylmalate dehydratase large subunit
UniProt: P0A6A6 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11576
Length: 466 (465)
Sequences: 3286
Seq/Len: 7.07
LEUC
Paralog alert: 0.83 [within 20: 0.04] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
280_Q 283_E 5.266 1.00
169_E 278_T 5.093 1.00
237_E 241_N 5.073 1.00
285_S 301_N 4.818 1.00
456_T 460_A 3.992 1.00
2_A 270_G 3.624 1.00
356_R 389_E 3.225 1.00
3_K 11_D 3.113 1.00
171_Q 278_T 2.986 1.00
238_T 241_N 2.967 1.00
167_K 204_C 2.966 1.00
173_K 210_D 2.797 1.00
180_A 215_G 2.775 1.00
425_A 445_V 2.759 1.00
342_V 450_A 2.752 1.00
360_E 363_K 2.677 1.00
167_K 276_V 2.652 1.00
45_R 92_E 2.578 1.00
393_K 397_E 2.561 1.00
204_C 276_V 2.548 1.00
169_E 276_V 2.515 1.00
355_L 386_A 2.406 1.00
253_K 257_D 2.345 1.00
171_Q 206_E 2.306 1.00
391_L 395_F 2.275 1.00
344_I 358_A 2.256 1.00
373_A 400_F 2.248 1.00
189_G 297_V 2.204 1.00
306_D 309_S 2.2 1.00
363_K 394_I 2.187 1.00
173_K 206_E 2.186 1.00
299_S 302_D 2.166 1.00
352_I 385_Q 2.165 1.00
174_A 183_I 2.16 1.00
261_Y 264_T 2.1 1.00
244_K 250_P 2.097 1.00
427_T 450_A 2.086 1.00
285_S 299_S 2.072 1.00
175_A 178_I 2.043 1.00
344_I 373_A 2.041 1.00
336_E 458_H 2.022 1.00
199_H 275_T 2.021 1.00
186_A 190_K 2.012 1.00
186_A 287_Q 2.008 1.00
316_R 320_E 1.958 1.00
338_A 458_H 1.926 1.00
27_I 123_T 1.926 1.00
190_K 279_L 1.919 1.00
207_A 281_A 1.903 1.00
168_I 201_V 1.876 1.00
201_V 226_M 1.867 1.00
342_V 425_A 1.831 1.00
383_K 387_E 1.827 1.00
51_V 153_L 1.809 1.00
383_K 405_P 1.803 1.00
9_L 232_L 1.788 1.00
80_R 84_Q 1.781 1.00
209_R 237_E 1.779 1.00
219_L 233_V 1.769 1.00
110_V 350_S 1.769 1.00
7_E 165_T 1.767 1.00
365_R 455_V 1.747 1.00
194_A 275_T 1.725 1.00
169_E 204_C 1.709 1.00
310_F 319_A 1.708 1.00
356_R 391_L 1.705 1.00
298_I 334_L 1.69 1.00
88_K 92_E 1.661 1.00
110_V 378_G 1.65 1.00
45_R 93_F 1.65 1.00
392_D 396_I 1.648 1.00
184_V 219_L 1.637 1.00
320_E 324_A 1.633 1.00
203_F 223_A 1.626 1.00
288_V 303_N 1.622 1.00
449_M 453_A 1.621 1.00
81_I 85_E 1.606 1.00
352_I 356_R 1.596 1.00
396_I 401_E 1.578 1.00
180_A 211_L 1.568 1.00
213_M 238_T 1.568 1.00
6_Y 163_A 1.561 1.00
392_D 402_W 1.556 1.00
254_D 257_D 1.556 1.00
357_A 448_A 1.553 1.00
140_L 230_A 1.553 1.00
166_M 275_T 1.552 1.00
170_V 281_A 1.548 1.00
287_Q 297_V 1.539 0.99
184_V 222_M 1.534 0.99
168_I 187_I 1.532 0.99
90_C 97_L 1.529 0.99
333_P 336_E 1.523 0.99
420_P 439_G 1.521 0.99
186_A 189_G 1.521 0.99
171_Q 280_Q 1.505 0.99
240_F 263_K 1.503 0.99
186_A 279_L 1.502 0.99
255_F 259_V 1.499 0.99
352_I 389_E 1.497 0.99
87_I 99_D 1.49 0.99
186_A 284_I 1.487 0.99
183_I 207_A 1.487 0.99
173_K 282_E 1.482 0.99
335_T 464_N 1.482 0.99
208_I 211_L 1.476 0.99
51_V 95_V 1.475 0.99
183_I 211_L 1.463 0.99
175_A 282_E 1.46 0.99
31_L 108_V 1.455 0.99
212_S 238_T 1.45 0.99
267_T 273_F 1.444 0.99
170_V 187_I 1.426 0.99
174_A 178_I 1.419 0.99
241_N 244_K 1.404 0.99
376_V 408_S 1.394 0.99
339_I 453_A 1.392 0.99
179_T 357_A 1.374 0.99
190_K 277_V 1.371 0.99
235_P 240_F 1.361 0.98
212_S 353_E 1.36 0.98
385_Q 389_E 1.359 0.98
118_T 134_H 1.345 0.98
387_E 392_D 1.344 0.98
208_I 219_L 1.343 0.98
58_F 117_V 1.341 0.98
367_V 373_A 1.336 0.98
339_I 371_V 1.332 0.98
207_A 211_L 1.327 0.98
393_K 396_I 1.321 0.98
174_A 210_D 1.319 0.98
170_V 183_I 1.317 0.98
234_A 266_Q 1.306 0.98
365_R 465_I 1.306 0.98
290_W 298_I 1.295 0.98
57_T 153_L 1.293 0.98
355_L 375_V 1.29 0.98
394_I 397_E 1.269 0.97
376_V 403_R 1.266 0.97
136_A 213_M 1.266 0.97
164_K 274_D 1.262 0.97
339_I 342_V 1.26 0.97
175_A 285_S 1.26 0.97
237_E 263_K 1.258 0.97
340_D 422_E 1.243 0.97
362_A 373_A 1.238 0.97
339_I 423_R 1.237 0.97
137_F 213_M 1.23 0.97
144_I 149_V 1.226 0.96
456_T 465_I 1.213 0.96
137_F 217_M 1.21 0.96
421_G 439_G 1.205 0.96
355_L 395_F 1.198 0.96
26_Y 157_T 1.195 0.96
132_A 223_A 1.19 0.96
342_V 427_T 1.187 0.96
317_A 320_E 1.18 0.95
418_L 424_C 1.179 0.95
302_D 305_P 1.177 0.95
363_K 397_E 1.175 0.95
306_D 310_F 1.174 0.95
449_M 462_I 1.171 0.95
235_P 256_D 1.17 0.95
220_C 233_V 1.168 0.95
103_P 384_A 1.159 0.95
341_K 422_E 1.148 0.94
29_R 117_V 1.146 0.94
237_E 244_K 1.141 0.94
32_V 108_V 1.139 0.94
313_P 317_A 1.138 0.94
215_G 353_E 1.137 0.94
338_A 370_G 1.133 0.94
209_R 266_Q 1.129 0.94
206_E 209_R 1.129 0.94
223_A 231_G 1.126 0.94
84_Q 88_K 1.126 0.94
118_T 137_F 1.126 0.94
327_G 330_P 1.125 0.94
171_Q 281_A 1.123 0.93
425_A 459_F 1.115 0.93
391_L 394_I 1.113 0.93
281_A 284_I 1.113 0.93
256_D 259_V 1.112 0.93
288_V 445_V 1.11 0.93
53_Q 56_K 1.107 0.93
206_E 210_D 1.1 0.92
300_V 449_M 1.099 0.92
200_V 230_A 1.094 0.92
423_R 441_R 1.091 0.92
412_A 417_R 1.09 0.92
170_V 207_A 1.088 0.92
27_I 152_V 1.087 0.92
61_M 97_L 1.082 0.92
356_R 385_Q 1.081 0.92
6_Y 230_A 1.074 0.91
61_M 99_D 1.073 0.91
359_A 391_L 1.071 0.91
70_K 419_N 1.071 0.91
384_A 388_A 1.067 0.91
343_F 428_S 1.067 0.91
240_F 244_K 1.064 0.91
183_I 206_E 1.062 0.91
364_G 397_E 1.062 0.91
74_A 77_E 1.061 0.91
361_I 451_A 1.06 0.91
455_V 465_I 1.058 0.90
169_E 206_E 1.054 0.90
445_V 449_M 1.052 0.90
110_V 379_S 1.051 0.90
339_I 425_A 1.048 0.90
43_G 150_E 1.041 0.89
36_T 411_L 1.041 0.89
289_T 297_V 1.04 0.89
179_T 448_A 1.039 0.89
445_V 459_F 1.037 0.89
259_V 263_K 1.036 0.89
342_V 454_A 1.036 0.89
388_A 393_K 1.034 0.89
348_T 429_N 1.033 0.89
206_E 282_E 1.03 0.89
32_V 57_T 1.024 0.88
267_T 270_G 1.023 0.88
425_A 453_A 1.02 0.88
6_Y 200_V 1.017 0.88
446_S 449_M 1.016 0.88
170_V 208_I 1.012 0.88
4_T 165_T 1.01 0.88
304_I 337_V 1.009 0.87
5_L 265_L 1.007 0.87
445_V 450_A 1.007 0.87
67_T 374_L 1.006 0.87
113_P 217_M 1.006 0.87
190_K 283_E 1.003 0.87
125_V 152_V 1.003 0.87
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1c96A10.93131000.149Contact Map0.687
2b3yA20.97641000.176Contact Map0.588
1l5jA20.89271000.217Contact Map0.69
1p3y110.371243.60.978Contact Map0.393
1tnsA10.122320.40.982Contact Map0.248
1su8A10.2103150.983Contact Map0.352
1g4dA10.1073120.984Contact Map0.258
1mvlA10.4013110.984Contact Map0.465
1qzuA40.39069.50.984Contact Map0.287
1jqkA60.21469.40.984Contact Map0.557
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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