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OPENSEQ.org
PUR5 - Phosphoribosylformylglycinamidine cyclo-ligase
UniProt: P08178 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10798
Length: 345 (342)
Sequences: 1725
Seq/Len: 5.04
PUR5
Paralog alert: 0.02 [within 20: 0.01] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
32_K 131_L 3.744 1.00
80_H 154_Y 3.491 1.00
189_P 197_V 3.387 1.00
200_I 289_V 3.206 1.00
128_E 132_Q 3.161 1.00
35_R 101_E 3.076 1.00
175_V 244_A 3.059 1.00
133_S 228_I 2.883 1.00
128_E 131_L 2.826 1.00
302_V 330_I 2.778 1.00
268_V 332_I 2.662 1.00
57_E 167_S 2.596 1.00
183_A 302_V 2.58 1.00
182_I 327_A 2.572 1.00
317_L 327_A 2.57 1.00
177_D 265_T 2.471 1.00
10_K 15_D 2.454 1.00
181_L 307_A 2.451 1.00
181_L 305_I 2.445 1.00
216_K 224_A 2.386 1.00
57_E 166_K 2.377 1.00
50_A 166_K 2.352 1.00
77_L 219_A 2.321 1.00
182_I 317_L 2.222 1.00
177_D 335_A 2.165 1.00
75_M 153_D 2.123 1.00
308_L 316_A 2.091 1.00
245_I 304_M 2.09 1.00
243_H 309_P 2.057 1.00
267_A 333_I 2.054 1.00
47_A 138_V 2.025 1.00
145_M 149_Y 1.958 1.00
200_I 283_L 1.958 1.00
21_A 25_R 1.941 1.00
309_P 312_E 1.917 1.00
79_R 220_D 1.908 1.00
260_V 307_A 1.907 1.00
268_V 334_K 1.887 1.00
25_R 120_S 1.86 1.00
189_P 194_Y 1.837 1.00
85_I 129_G 1.801 1.00
164_V 169_I 1.765 1.00
71_L 148_M 1.759 1.00
95_L 160_C 1.748 1.00
181_L 267_A 1.731 1.00
56_R 167_S 1.716 1.00
129_G 228_I 1.713 1.00
216_K 221_H 1.708 1.00
132_Q 228_I 1.705 1.00
253_F 257_I 1.704 1.00
174_K 243_H 1.702 1.00
266_Q 334_K 1.702 1.00
317_L 329_K 1.671 0.99
179_D 243_H 1.659 0.99
25_R 117_D 1.628 0.99
275_Q 281_N 1.604 0.99
56_R 165_E 1.604 0.99
233_V 304_M 1.595 0.99
63_G 95_L 1.592 0.99
59_V 169_I 1.59 0.99
83_I 156_V 1.564 0.99
271_E 291_H 1.562 0.99
313_V 317_L 1.551 0.99
232_S 325_E 1.549 0.99
240_V 316_A 1.547 0.99
81_D 124_S 1.543 0.99
221_H 224_A 1.543 0.99
295_Y 300_C 1.528 0.99
20_N 255_E 1.525 0.99
119_A 122_V 1.522 0.99
50_A 57_E 1.522 0.99
176_S 309_P 1.513 0.99
73_L 77_L 1.481 0.98
313_V 329_K 1.481 0.98
17_D 296_R 1.464 0.98
109_Y 119_A 1.462 0.98
85_I 132_Q 1.461 0.98
29_V 124_S 1.445 0.98
111_T 119_A 1.445 0.98
236_L 316_A 1.437 0.98
244_A 260_V 1.433 0.98
22_L 26_I 1.429 0.98
175_V 333_I 1.423 0.98
239_K 315_K 1.4 0.97
187_S 224_A 1.394 0.97
276_W 281_N 1.392 0.97
261_L 265_T 1.391 0.97
37_E 49_C 1.39 0.97
253_F 305_I 1.39 0.97
22_L 119_A 1.389 0.97
312_E 315_K 1.375 0.97
124_S 128_E 1.374 0.97
24_G 27_K 1.371 0.97
97_V 245_I 1.367 0.97
271_E 295_Y 1.365 0.97
72_R 153_D 1.361 0.97
289_V 294_M 1.352 0.97
284_Q 294_M 1.348 0.97
18_A 116_V 1.343 0.96
35_R 49_C 1.336 0.96
78_K 118_T 1.335 0.96
90_M 226_T 1.333 0.96
90_M 191_S 1.318 0.96
175_V 260_V 1.317 0.96
19_G 23_V 1.312 0.96
314_D 318_A 1.312 0.96
306_I 316_A 1.308 0.96
180_V 313_V 1.3 0.96
29_V 120_S 1.298 0.95
315_K 318_A 1.297 0.95
181_L 269_I 1.296 0.95
180_V 310_A 1.296 0.95
294_M 298_F 1.295 0.95
235_E 323_N 1.276 0.95
216_K 220_D 1.275 0.95
185_G 328_W 1.272 0.95
46_G 61_V 1.265 0.95
290_E 293_E 1.264 0.95
287_G 294_M 1.256 0.94
165_E 168_E 1.241 0.94
227_R 325_E 1.239 0.94
284_Q 291_H 1.235 0.94
191_S 226_T 1.235 0.94
39_M 59_V 1.228 0.93
182_I 329_K 1.224 0.93
233_V 306_I 1.221 0.93
257_I 269_I 1.208 0.93
217_P 220_D 1.193 0.92
275_Q 278_E 1.193 0.92
118_T 152_E 1.192 0.92
59_V 166_K 1.178 0.91
58_P 165_E 1.178 0.91
235_E 238_E 1.173 0.91
109_Y 122_V 1.173 0.91
232_S 235_E 1.17 0.91
184_L 304_M 1.148 0.90
129_G 132_Q 1.144 0.90
180_V 329_K 1.139 0.89
62_S 104_F 1.137 0.89
310_A 332_I 1.137 0.89
245_I 306_I 1.135 0.89
183_A 275_Q 1.126 0.89
96_V 304_M 1.122 0.89
59_V 164_V 1.112 0.88
21_A 24_G 1.11 0.88
37_E 101_E 1.104 0.87
104_F 161_V 1.103 0.87
236_L 320_L 1.101 0.87
9_Y 14_V 1.083 0.86
62_S 161_V 1.079 0.86
321_N 326_N 1.075 0.85
239_K 319_L 1.075 0.85
204_S 286_A 1.069 0.85
56_R 168_E 1.067 0.85
91_C 160_C 1.063 0.85
55_Y 163_V 1.061 0.84
254_W 257_I 1.06 0.84
201_L 222_L 1.058 0.84
185_G 327_A 1.048 0.83
8_S 11_D 1.046 0.83
182_I 308_L 1.044 0.83
280_F 294_M 1.043 0.83
9_Y 13_G 1.041 0.83
129_G 133_S 1.035 0.82
83_I 122_V 1.031 0.82
314_D 329_K 1.027 0.82
134_G 234_L 1.025 0.82
104_F 138_V 1.022 0.81
114_L 119_A 1.019 0.81
318_A 322_A 1.019 0.81
61_V 100_A 1.017 0.81
79_R 219_A 1.009 0.80
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3kizA20.94491000.432Contact Map0.609
3mdoA20.93621000.448Contact Map0.632
2btuA20.97681000.464Contact Map0.752
2z01A10.97681000.466Contact Map0.772
3p4eA10.99711000.47Contact Map0.715
3m84A20.97971000.477Contact Map0.758
2v9yA20.90721000.524Contact Map0.759
2z1eA10.89571000.666Contact Map0.636
3u0oA20.91881000.668Contact Map0.593
3ugjA10.93911000.671Contact Map0.457
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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