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OPENSEQ.org
GLGB - 1,4-alpha-glucan branching enzyme GlgB
UniProt: P07762 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10378
Length: 728 (717)
Sequences: 1560
Seq/Len: 2.18
GLGB
Paralog alert: 0.10 [within 20: 0.01] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
605_D 686_Y 5.497 1.00
318_F 322_I 4.614 1.00
266_R 317_D 4.431 1.00
559_A 641_F 3.903 1.00
447_G 478_W 3.802 1.00
266_R 320_Y 3.477 1.00
559_A 722_W 3.268 1.00
627_E 707_R 3.179 1.00
392_Y 397_F 3.169 1.00
284_L 325_A 3.135 1.00
343_T 366_Y 2.946 1.00
669_I 711_L 2.941 1.00
573_A 599_V 2.895 1.00
161_G 191_E 2.878 1.00
511_I 515_Y 2.825 1.00
120_R 123_E 2.821 1.00
226_K 323_D 2.793 1.00
254_R 271_Q 2.76 1.00
655_S 720_T 2.754 1.00
137_G 178_F 2.748 1.00
153_V 201_L 2.698 1.00
319_R 396_R 2.664 1.00
555_G 720_T 2.661 1.00
245_I 520_V 2.638 1.00
510_G 560_F 2.616 1.00
638_V 715_L 2.57 1.00
677_E 693_N 2.557 1.00
166_M 177_L 2.554 1.00
286_L 321_F 2.53 1.00
501_Q 635_E 2.508 1.00
270_D 324_A 2.502 1.00
418_K 421_E 2.435 1.00
523_L 570_N 2.404 1.00
629_L 652_I 2.396 1.00
637_S 658_T 2.394 1.00
369_D 416_S 2.388 1.00
317_D 320_Y 2.378 1.00
633_D 636_R 2.378 1.00
182_A 186_Q 2.367 1.00
699_S 709_H 2.356 1.00
613_H 724_V 2.35 1.00
155_Q 186_Q 2.331 1.00
666_R 710_S 2.305 1.00
248_V 276_A 2.284 1.00
228_V 398_G 2.282 1.00
278_W 604_R 2.279 1.00
627_E 644_R 2.243 1.00
206_Y 357_Y 2.23 1.00
316_D 319_R 2.209 1.00
183_H 186_Q 2.203 1.00
559_A 653_V 2.197 1.00
131_T 136_T 2.191 1.00
572_F 584_L 2.097 0.99
446_L 453_A 2.097 0.99
321_F 325_A 2.085 0.99
507_L 639_L 2.058 0.99
211_Q 215_E 2.044 0.99
182_A 220_I 2.044 0.99
60_L 71_G 2.036 0.99
579_N 582_A 2.018 0.99
535_I 554_Y 2.018 0.99
320_Y 324_A 2.013 0.99
437_E 440_R 2.005 0.99
324_A 327_A 1.999 0.99
583_S 587_H 1.962 0.99
427_F 433_L 1.924 0.99
666_R 700_D 1.919 0.99
618_E 646_K 1.905 0.99
109_D 118_H 1.902 0.99
121_P 206_Y 1.893 0.99
602_L 681_T 1.892 0.99
286_L 289_I 1.891 0.99
566_L 570_N 1.889 0.99
270_D 320_Y 1.878 0.99
444_R 470_Q 1.862 0.98
274_P 328_A 1.826 0.98
138_T 182_A 1.824 0.98
276_A 284_L 1.816 0.98
383_S 441_N 1.811 0.98
277_K 328_A 1.81 0.98
230_T 398_G 1.792 0.98
306_Y 389_N 1.775 0.98
324_A 328_A 1.766 0.98
276_A 330_L 1.763 0.98
364_E 413_R 1.749 0.97
213_R 295_D 1.733 0.97
713_L 723_L 1.727 0.97
243_I 454_V 1.719 0.97
692_G 695_G 1.712 0.97
661_P 691_A 1.711 0.97
261_F 267_E 1.701 0.97
290_N 389_N 1.695 0.97
269_A 320_Y 1.689 0.97
677_E 723_L 1.684 0.97
318_F 399_I 1.676 0.96
210_A 307_A 1.669 0.96
654_A 713_L 1.669 0.96
229_Q 323_D 1.658 0.96
542_D 545_Q 1.636 0.96
442_T 455_T 1.625 0.95
289_I 318_F 1.625 0.95
108_M 111_W 1.618 0.95
414_D 430_R 1.617 0.95
677_E 695_G 1.603 0.95
266_R 269_A 1.595 0.95
203_S 210_A 1.583 0.95
273_V 325_A 1.581 0.95
672_P 699_S 1.579 0.94
273_V 328_A 1.572 0.94
543_A 575_G 1.565 0.94
273_V 324_A 1.562 0.94
110_A 174_I 1.558 0.94
378_G 434_E 1.555 0.94
191_E 201_L 1.554 0.94
194_D 200_R 1.546 0.94
436_I 463_F 1.53 0.93
493_M 657_F 1.527 0.93
555_G 681_T 1.525 0.93
349_A 373_L 1.524 0.93
523_L 553_Y 1.513 0.93
684_M 690_N 1.513 0.93
226_K 229_Q 1.512 0.93
109_D 112_L 1.51 0.93
676_R 726_E 1.503 0.92
308_P 318_F 1.501 0.92
630_V 640_I 1.501 0.92
563_K 620_D 1.492 0.92
367_H 374_I 1.489 0.92
664_D 712_S 1.482 0.92
669_I 675_W 1.479 0.92
270_D 274_P 1.475 0.91
359_H 376_N 1.475 0.91
229_Q 327_A 1.469 0.91
487_H 491_D 1.468 0.91
111_W 115_E 1.463 0.91
323_D 327_A 1.461 0.91
642_V 652_I 1.461 0.91
276_A 280_G 1.452 0.91
683_S 690_N 1.451 0.91
702_I 710_S 1.449 0.90
554_Y 566_L 1.441 0.90
674_K 696_T 1.435 0.90
363_R 423_I 1.435 0.90
305_L 375_Y 1.433 0.90
164_H 179_I 1.433 0.90
609_T 678_I 1.43 0.90
392_Y 396_R 1.423 0.89
314_T 317_D 1.422 0.89
700_D 710_S 1.418 0.89
495_L 499_Y 1.417 0.89
489_T 553_Y 1.406 0.89
209_E 221_C 1.403 0.88
106_Q 109_D 1.402 0.88
543_A 546_K 1.397 0.88
155_Q 187_L 1.395 0.88
495_L 506_K 1.395 0.88
703_A 710_S 1.386 0.88
439_L 477_F 1.386 0.88
284_L 321_F 1.381 0.87
582_A 585_D 1.379 0.87
597_H 601_R 1.377 0.87
333_I 456_M 1.377 0.87
138_T 183_H 1.368 0.87
663_H 714_T 1.367 0.87
187_L 217_A 1.367 0.87
487_H 715_L 1.365 0.87
651_I 722_W 1.353 0.86
120_R 449_Q 1.348 0.86
658_T 662_R 1.347 0.86
233_R 452_G 1.329 0.85
645_D 649_N 1.327 0.84
609_T 679_L 1.326 0.84
148_R 195_A 1.324 0.84
384_N 388_G 1.322 0.84
208_F 223_L 1.321 0.84
666_R 702_I 1.318 0.84
151_S 191_E 1.313 0.84
661_P 717_P 1.309 0.83
367_H 409_S 1.306 0.83
533_K 537_D 1.306 0.83
251_G 312_F 1.305 0.83
308_P 392_Y 1.301 0.83
250_L 265_Y 1.294 0.82
86_V 95_I 1.294 0.82
552_A 720_T 1.293 0.82
491_D 506_K 1.291 0.82
146_N 354_T 1.289 0.82
668_G 710_S 1.287 0.82
296_G 580_H 1.287 0.82
466_V 475_L 1.286 0.82
157_N 164_H 1.279 0.81
128_H 208_F 1.277 0.81
279_M 600_Q 1.273 0.81
118_H 121_P 1.271 0.81
446_L 455_T 1.269 0.81
237_N 240_D 1.268 0.81
652_I 669_I 1.263 0.80
250_L 268_L 1.26 0.80
124_T 139_R 1.259 0.80
410_M 435_A 1.25 0.79
676_R 724_V 1.249 0.79
274_P 325_A 1.247 0.79
394_I 446_L 1.246 0.79
291_E 309_T 1.241 0.79
444_R 471_D 1.236 0.78
544_W 661_P 1.236 0.78
138_T 220_I 1.235 0.78
289_I 334_L 1.234 0.78
48_V 60_L 1.231 0.78
306_Y 388_G 1.23 0.78
666_R 712_S 1.229 0.78
290_N 302_P 1.226 0.77
537_D 546_K 1.224 0.77
479_Y 621_F 1.216 0.77
250_L 274_P 1.213 0.76
678_I 721_I 1.209 0.76
555_G 655_S 1.206 0.76
13_L 68_F 1.203 0.75
147_A 352_D 1.201 0.75
152_V 179_I 1.191 0.74
652_I 711_L 1.191 0.74
653_V 722_W 1.19 0.74
148_R 193_I 1.19 0.74
250_L 286_L 1.185 0.74
9_V 18_F 1.184 0.74
649_N 724_V 1.18 0.73
678_I 724_V 1.176 0.73
213_R 262_W 1.174 0.73
278_W 600_Q 1.174 0.73
44_T 89_H 1.17 0.73
112_L 115_E 1.168 0.72
365_G 376_N 1.168 0.72
605_D 685_H 1.165 0.72
387_V 442_T 1.161 0.72
11_N 15_A 1.16 0.72
651_I 678_I 1.16 0.72
629_L 668_G 1.155 0.71
395_E 449_Q 1.152 0.71
443_N 455_T 1.145 0.70
366_Y 371_N 1.142 0.70
96_D 100_R 1.14 0.70
680_N 693_N 1.139 0.70
673_G 699_S 1.139 0.70
478_W 518_N 1.138 0.70
672_P 709_H 1.135 0.69
376_N 379_R 1.131 0.69
164_H 177_L 1.13 0.69
390_A 402_L 1.13 0.69
274_P 324_A 1.129 0.69
88_W 93_N 1.129 0.69
208_F 392_Y 1.127 0.68
20_D 23_S 1.124 0.68
544_W 689_S 1.124 0.68
510_G 556_W 1.123 0.68
36_V 48_V 1.122 0.68
12_A 19_A 1.12 0.68
112_L 118_H 1.118 0.68
123_E 396_R 1.113 0.67
496_D 499_Y 1.112 0.67
491_D 494_K 1.111 0.67
13_L 24_V 1.107 0.66
374_I 409_S 1.107 0.66
636_R 665_Y 1.105 0.66
264_S 267_E 1.104 0.66
232_E 236_A 1.102 0.66
426_E 437_E 1.102 0.66
553_Y 556_W 1.102 0.66
505_D 634_K 1.097 0.65
619_L 622_D 1.096 0.65
150_V 192_M 1.095 0.65
662_R 715_L 1.091 0.65
343_T 371_N 1.086 0.64
699_S 711_L 1.085 0.64
226_K 327_A 1.083 0.64
148_R 197_G 1.081 0.64
359_H 365_G 1.079 0.64
186_Q 220_I 1.078 0.63
30_T 33_G 1.077 0.63
121_P 388_G 1.076 0.63
296_G 581_D 1.074 0.63
395_E 450_V 1.073 0.63
677_E 683_S 1.072 0.63
365_G 374_I 1.07 0.63
290_N 399_I 1.069 0.63
232_E 329_G 1.065 0.62
255_R 583_S 1.064 0.62
281_F 566_L 1.062 0.62
243_I 283_H 1.061 0.62
125_L 141_S 1.061 0.62
117_T 441_N 1.058 0.61
559_A 655_S 1.052 0.61
640_I 654_A 1.051 0.61
263_L 271_Q 1.051 0.61
248_V 286_L 1.05 0.60
40_L 46_V 1.049 0.60
550_L 554_Y 1.049 0.60
683_S 692_G 1.047 0.60
239_F 621_F 1.042 0.60
269_A 324_A 1.042 0.60
193_I 199_L 1.038 0.59
149_R 165_P 1.038 0.59
45_D 87_V 1.037 0.59
149_R 193_I 1.036 0.59
131_T 134_G 1.035 0.59
360_S 364_E 1.035 0.59
364_E 423_I 1.033 0.59
119_L 387_V 1.033 0.59
601_R 686_Y 1.032 0.58
590_E 597_H 1.031 0.58
454_V 478_W 1.03 0.58
254_R 263_L 1.029 0.58
590_E 593_D 1.029 0.58
492_Y 504_H 1.028 0.58
697_V 711_L 1.028 0.58
179_I 182_A 1.028 0.58
630_V 633_D 1.025 0.58
515_Y 619_L 1.024 0.58
281_F 558_W 1.024 0.58
545_Q 661_P 1.022 0.57
210_A 304_G 1.021 0.57
639_L 657_F 1.021 0.57
576_R 585_D 1.019 0.57
169_R 176_E 1.016 0.57
677_E 692_G 1.013 0.56
608_L 612_H 1.013 0.56
184_N 222_G 1.011 0.56
367_H 377_Y 1.007 0.56
270_D 273_V 1.006 0.56
37_R 70_S 1.006 0.56
13_L 39_L 1.006 0.56
601_R 605_D 1.005 0.55
302_P 305_L 1.004 0.55
281_F 571_E 1.001 0.55
47_W 59_K 1 0.55
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3k1dA10.9781000.273Contact Map0.735
1m7xA40.84341000.436Contact Map0.794
3amlA10.86541000.459Contact Map0.657
4bzyA30.89561000.504Contact Map0.693
1bf2A10.79671000.611Contact Map0.536
2e8yA20.84891000.614Contact Map0.647
1wzlA20.73351000.625Contact Map0.581
2bhuA10.72391000.629Contact Map0.604
3fawA10.91071000.631Contact Map0.573
3m07A10.7391000.633Contact Map0.662
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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