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OPENSEQ.org
DLD - D-lactate dehydrogenase
UniProt: P06149 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10231
Length: 571 (499)
Sequences: 1491
Seq/Len: 2.99
DLD
Paralog alert: 0.58 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: DLD GLCD GLCE YGCU
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
119_Y 264_A 4.506 1.00
155_I 265_V 4.504 1.00
472_H 520_Q 4.212 1.00
116_V 267_L 4.179 1.00
156_C 256_C 3.846 1.00
111_G 114_E 3.795 1.00
15_E 67_T 3.644 1.00
106_K 119_Y 3.514 1.00
62_L 72_I 3.487 1.00
285_Q 288_V 3.037 1.00
472_H 521_R 2.921 1.00
479_S 513_Q 2.921 1.00
251_F 261_A 2.876 1.00
28_D 31_K 2.821 1.00
99_I 262_V 2.721 1.00
69_D 551_T 2.542 1.00
65_C 97_V 2.529 1.00
50_V 97_V 2.498 1.00
136_R 269_T 2.468 1.00
109_V 117_L 2.44 1.00
195_L 251_F 2.406 1.00
107_L 122_T 2.383 1.00
115_Q 136_R 2.362 1.00
12_F 64_A 2.347 1.00
463_R 466_D 2.332 1.00
50_V 61_V 2.322 1.00
480_Q 513_Q 2.315 1.00
194_D 264_A 2.296 1.00
155_I 263_F 2.294 1.00
64_A 68_A 2.283 1.00
117_L 264_A 2.271 1.00
15_E 68_A 2.27 1.00
110_L 130_A 2.199 1.00
59_W 202_I 2.194 1.00
287_E 471_E 2.14 1.00
283_T 292_I 2.111 1.00
66_V 551_T 2.107 1.00
118_A 152_I 2.088 1.00
198_T 554_M 2.012 1.00
27_T 48_A 2.007 1.00
126_S 129_K 1.96 1.00
191_L 268_D 1.952 1.00
12_F 16_L 1.904 0.99
116_V 127_L 1.903 0.99
196_G 202_I 1.874 0.99
55_L 202_I 1.859 0.99
306_A 431_F 1.857 0.99
26_L 31_K 1.841 0.99
400_G 403_E 1.835 0.99
55_L 194_D 1.818 0.99
25_L 50_V 1.817 0.99
197_E 201_Q 1.774 0.99
58_L 101_T 1.773 0.99
284_N 288_V 1.766 0.99
14_N 17_A 1.754 0.99
108_H 119_Y 1.731 0.99
75_Q 78_N 1.7 0.98
53_G 57_E 1.695 0.98
287_E 291_E 1.691 0.98
251_F 263_F 1.685 0.98
20_V 50_V 1.674 0.98
55_L 59_W 1.665 0.98
61_V 99_I 1.647 0.98
401_V 405_K 1.629 0.98
128_E 137_E 1.608 0.97
468_E 521_R 1.596 0.97
122_T 127_L 1.581 0.97
198_T 548_N 1.57 0.97
131_L 269_T 1.568 0.97
192_G 206_L 1.563 0.97
60_R 63_K 1.552 0.97
19_L 64_A 1.543 0.96
305_V 397_A 1.542 0.96
425_E 429_K 1.537 0.96
76_A 151_V 1.521 0.96
461_A 524_Q 1.518 0.96
15_E 64_A 1.5 0.96
59_W 196_G 1.499 0.96
475_P 479_S 1.498 0.96
117_L 266_R 1.496 0.96
62_L 554_M 1.483 0.95
190_H 254_S 1.449 0.94
53_G 103_R 1.442 0.94
13_L 48_A 1.441 0.94
250_L 544_F 1.438 0.94
13_L 17_A 1.435 0.94
288_V 291_E 1.422 0.94
128_E 131_L 1.421 0.94
468_E 472_H 1.418 0.94
49_V 98_I 1.412 0.93
16_L 50_V 1.393 0.93
12_F 70_K 1.391 0.93
460_I 473_L 1.38 0.92
17_A 22_S 1.375 0.92
101_T 120_P 1.373 0.92
192_G 266_R 1.363 0.92
79_T 461_A 1.363 0.92
543_K 547_E 1.356 0.91
54_S 57_E 1.35 0.91
114_E 266_R 1.35 0.91
72_I 99_I 1.331 0.90
287_E 468_E 1.323 0.90
112_K 134_L 1.321 0.90
295_H 414_Q 1.32 0.90
526_P 531_V 1.319 0.90
400_G 404_A 1.314 0.90
21_G 24_H 1.312 0.90
297_L 304_P 1.305 0.89
281_I 394_L 1.304 0.89
194_D 204_S 1.304 0.89
314_I 437_A 1.298 0.89
125_Y 145_S 1.294 0.89
489_H 528_E 1.292 0.89
260_L 544_F 1.287 0.88
123_T 126_S 1.283 0.88
195_L 261_A 1.28 0.88
508_H 512_E 1.271 0.88
228_Y 259_K 1.27 0.87
423_T 426_E 1.267 0.87
111_G 266_R 1.258 0.87
46_A 78_N 1.245 0.86
17_A 25_L 1.245 0.86
393_L 430_A 1.243 0.86
116_V 130_A 1.242 0.86
155_I 170_Y 1.236 0.85
115_Q 271_E 1.235 0.85
223_A 227_D 1.234 0.85
14_N 18_R 1.233 0.85
26_L 51_F 1.23 0.85
256_C 260_L 1.224 0.85
114_E 465_N 1.205 0.83
309_Y 490_F 1.196 0.83
516_E 520_Q 1.195 0.83
80_G 84_G 1.191 0.82
312_R 316_D 1.19 0.82
213_D 218_H 1.178 0.82
118_A 127_L 1.174 0.81
145_S 435_F 1.173 0.81
482_V 501_V 1.167 0.81
256_C 554_M 1.164 0.80
55_L 196_G 1.158 0.80
192_G 204_S 1.157 0.80
508_H 537_A 1.156 0.80
456_L 511_K 1.155 0.80
308_E 434_R 1.153 0.80
74_M 101_T 1.151 0.79
24_H 53_G 1.144 0.79
46_A 75_Q 1.144 0.79
108_H 117_L 1.142 0.79
35_Y 51_F 1.141 0.79
533_H 558_I 1.14 0.78
319_E 375_S 1.13 0.78
239_T 544_F 1.13 0.78
130_A 133_P 1.129 0.78
224_H 233_R 1.128 0.77
475_P 478_D 1.126 0.77
108_H 264_A 1.12 0.77
63_K 67_T 1.119 0.77
220_G 224_H 1.119 0.77
58_L 99_I 1.108 0.76
311_H 486_Y 1.104 0.75
73_L 91_D 1.101 0.75
152_I 155_I 1.099 0.75
26_L 35_Y 1.098 0.75
247_P 548_N 1.094 0.74
64_A 70_K 1.091 0.74
57_E 60_R 1.087 0.74
406_S 409_V 1.084 0.73
410_D 475_P 1.083 0.73
132_K 272_A 1.08 0.73
19_L 60_R 1.08 0.73
406_S 410_D 1.077 0.73
194_D 202_I 1.077 0.73
107_L 130_A 1.076 0.73
12_F 68_A 1.076 0.73
457_A 498_D 1.075 0.73
110_L 116_V 1.068 0.72
16_L 70_K 1.067 0.72
15_E 19_L 1.066 0.72
74_M 262_V 1.062 0.71
129_K 132_K 1.061 0.71
123_T 144_S 1.059 0.71
44_G 87_P 1.059 0.71
53_G 63_K 1.052 0.70
473_L 477_I 1.049 0.70
480_Q 509_A 1.048 0.70
56_L 60_R 1.048 0.70
308_E 528_E 1.039 0.69
70_K 553_S 1.038 0.69
105_D 126_S 1.034 0.68
368_K 371_H 1.033 0.68
209_D 212_K 1.033 0.68
192_G 264_A 1.032 0.68
61_V 107_L 1.029 0.68
66_V 69_D 1.027 0.68
29_P 32_T 1.026 0.67
59_W 62_L 1.025 0.67
55_L 104_L 1.023 0.67
55_L 58_L 1.023 0.67
420_F 430_A 1.023 0.67
70_K 97_V 1.022 0.67
285_Q 291_E 1.022 0.67
71_I 93_D 1.022 0.67
51_F 102_L 1.018 0.67
102_L 151_V 1.014 0.66
398_G 403_E 1.014 0.66
24_H 57_E 1.011 0.66
475_P 546_R 1.01 0.66
388_Y 417_G 1.005 0.65
29_P 33_A 1.003 0.65
476_E 517_L 1.001 0.65
466_D 522_G 1 0.65
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1f0xA20.99651000.271Contact Map0.54
3pm9A60.77231000.435Contact Map0.778
2uuuA40.80911000.445Contact Map0.703
4bbyA40.81441000.484Contact Map0.664
1wvfA10.82311000.508Contact Map0.685
2exrA10.75661000.525Contact Map0.486
1e8gA20.82661000.53Contact Map0.637
1w1oA10.75311000.542Contact Map0.591
4fehA10.77231000.633Contact Map0.632
1uxyA10.58321000.724Contact Map0.601
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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