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OPENSEQ.org
PNP - Polyribonucleotide nucleotidyltransferase
UniProt: P05055 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10743
Length: 711 (692)
Sequences: 1317
Seq/Len: 1.90
PNP
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
253_R 276_E 4.88 1.00
536_V 539_Q 4.646 1.00
354_A 449_A 4.536 1.00
320_E 323_M 4.072 1.00
459_A 500_D 3.995 1.00
183_E 220_N 3.897 1.00
654_I 666_L 3.798 1.00
560_I 568_V 3.711 1.00
22_M 330_R 3.516 1.00
322_D 528_G 3.455 1.00
628_K 671_E 3.416 1.00
626_T 671_E 3.353 1.00
161_Y 203_G 3.292 1.00
42_V 133_G 3.127 1.00
626_T 673_P 3.031 1.00
597_I 609_I 3.004 1.00
499_R 535_G 2.914 1.00
42_V 115_A 2.885 1.00
114_I 393_V 2.846 1.00
464_I 479_S 2.839 1.00
244_A 248_A 2.728 1.00
311_A 474_N 2.699 1.00
327_L 536_V 2.586 1.00
211_Q 214_V 2.571 1.00
350_A 431_I 2.545 1.00
156_Y 174_K 2.524 1.00
254_L 270_V 2.427 1.00
247_A 303_N 2.412 1.00
332_G 452_D 2.404 1.00
250_A 277_T 2.399 1.00
324_I 479_S 2.368 1.00
158_N 172_E 2.364 1.00
411_V 446_A 2.358 1.00
374_D 408_K 2.342 1.00
543_A 546_G 2.33 1.00
667_Q 670_Q 2.327 1.00
602_G 606_K 2.31 1.00
417_D 420_K 2.299 1.00
571_K 574_S 2.295 1.00
156_Y 200_Q 2.288 1.00
630_T 640_A 2.285 1.00
578_A 582_E 2.284 1.00
157_I 162_V 2.275 1.00
275_S 279_A 2.267 1.00
556_H 605_A 2.233 1.00
630_T 646_E 2.227 1.00
252_A 256_D 2.225 1.00
166_T 169_E 2.194 0.99
262_D 265_E 2.165 0.99
479_S 529_A 2.15 0.99
269_Q 272_V 2.09 0.99
415_M 425_V 2.082 0.99
123_Q 168_D 2.071 0.99
499_R 538_E 2.045 0.99
475_Y 521_V 2.043 0.99
559_K 594_T 2.04 0.99
116_T 385_Y 2.018 0.99
649_V 654_I 2.001 0.99
217_Q 221_E 1.981 0.99
16_V 35_M 1.97 0.99
606_K 609_I 1.967 0.99
36_D 121_N 1.953 0.99
524_N 528_G 1.947 0.99
154_V 163_L 1.945 0.99
352_V 445_G 1.943 0.99
160_Q 169_E 1.936 0.98
678_E 686_R 1.935 0.98
76_S 79_R 1.921 0.98
391_G 394_G 1.918 0.98
620_E 623_R 1.916 0.98
516_K 520_Q 1.913 0.98
414_V 457_I 1.913 0.98
447_S 460_A 1.908 0.98
25_R 433_E 1.899 0.98
607_H 610_R 1.898 0.98
336_R 551_F 1.891 0.98
527_K 531_L 1.889 0.98
18_L 132_I 1.867 0.98
18_L 33_V 1.851 0.98
186_V 205_V 1.846 0.98
140_L 226_A 1.841 0.98
33_V 133_G 1.834 0.98
410_G 504_A 1.817 0.97
676_V 685_I 1.813 0.97
139_S 223_V 1.798 0.97
609_I 613_E 1.79 0.97
155_G 173_S 1.775 0.97
452_D 545_R 1.763 0.97
460_A 538_E 1.756 0.97
556_H 609_I 1.749 0.97
179_V 208_G 1.738 0.96
639_V 649_V 1.729 0.96
554_R 599_A 1.72 0.96
568_V 595_V 1.711 0.96
183_E 217_Q 1.694 0.96
8_K 17_T 1.691 0.96
651_I 662_V 1.691 0.96
168_D 171_K 1.681 0.95
597_I 612_I 1.663 0.95
654_I 659_V 1.66 0.95
23_M 432_T 1.651 0.95
8_K 15_T 1.65 0.95
558_I 609_I 1.647 0.95
321_K 476_V 1.641 0.95
156_Y 161_Y 1.636 0.94
647_G 687_L 1.633 0.94
197_S 502_I 1.632 0.94
329_V 540_A 1.631 0.94
132_I 215_V 1.63 0.94
307_S 311_A 1.629 0.94
582_E 611_R 1.624 0.94
354_A 427_V 1.622 0.94
447_S 538_E 1.618 0.94
240_E 244_A 1.611 0.94
131_M 149_I 1.605 0.94
257_A 273_I 1.605 0.94
641_I 685_I 1.587 0.93
538_E 542_N 1.587 0.93
253_R 256_D 1.583 0.93
272_V 276_E 1.58 0.93
353_T 430_E 1.567 0.93
590_E 594_T 1.565 0.93
310_L 474_N 1.563 0.92
606_K 610_R 1.544 0.92
459_A 499_R 1.542 0.92
146_N 512_E 1.533 0.92
384_P 393_V 1.527 0.91
619_I 676_V 1.521 0.91
206_V 516_K 1.514 0.91
249_L 280_T 1.506 0.91
631_R 684_R 1.506 0.91
28_T 142_G 1.499 0.90
215_V 219_I 1.494 0.90
31_V 136_A 1.492 0.90
99_I 138_L 1.491 0.90
7_R 225_E 1.49 0.90
127_D 153_R 1.486 0.90
610_R 614_E 1.484 0.90
268_A 272_V 1.478 0.89
603_E 607_H 1.474 0.89
649_V 692_A 1.473 0.89
575_V 615_I 1.467 0.89
661_K 664_D 1.459 0.89
640_A 646_E 1.455 0.88
558_I 595_V 1.455 0.88
649_V 666_L 1.444 0.88
33_V 129_V 1.435 0.88
191_S 505_L 1.428 0.87
624_V 692_A 1.426 0.87
354_A 453_A 1.421 0.87
568_V 612_I 1.42 0.87
656_D 692_A 1.418 0.87
153_R 176_D 1.418 0.87
105_E 302_K 1.418 0.87
18_L 136_A 1.399 0.86
274_K 294_G 1.398 0.86
653_Q 691_E 1.393 0.85
168_D 172_E 1.39 0.85
324_I 532_H 1.388 0.85
558_I 597_I 1.386 0.85
440_M 443_V 1.379 0.85
470_K 511_I 1.377 0.84
39_A 335_P 1.371 0.84
51_K 54_Q 1.365 0.84
567_D 615_I 1.356 0.83
672_V 689_I 1.356 0.83
583_T 607_H 1.354 0.83
34_S 37_D 1.352 0.83
305_V 309_V 1.348 0.83
273_I 276_E 1.339 0.82
174_K 200_Q 1.326 0.81
247_A 251_E 1.323 0.81
154_V 207_F 1.322 0.81
223_V 227_G 1.318 0.81
46_G 231_W 1.317 0.81
655_A 689_I 1.316 0.81
617_A 623_R 1.316 0.81
174_K 194_Q 1.314 0.80
245_R 249_L 1.313 0.80
634_D 663_T 1.309 0.80
175_L 196_L 1.307 0.80
316_I 484_D 1.296 0.79
532_H 536_V 1.292 0.79
610_R 613_E 1.29 0.79
87_G 91_I 1.289 0.79
688_S 691_E 1.286 0.78
246_V 250_A 1.283 0.78
568_V 575_V 1.278 0.78
595_V 612_I 1.277 0.78
131_M 151_A 1.276 0.78
329_V 536_V 1.275 0.78
378_F 446_A 1.273 0.77
584_G 604_K 1.27 0.77
107_F 231_W 1.269 0.77
46_G 143_I 1.265 0.77
161_Y 200_Q 1.261 0.77
107_F 111_V 1.255 0.76
163_L 207_F 1.255 0.76
150_G 514_I 1.252 0.76
136_A 222_L 1.251 0.76
46_G 111_V 1.25 0.76
451_M 457_I 1.243 0.75
39_A 334_L 1.242 0.75
46_G 107_F 1.241 0.75
601_D 604_K 1.235 0.74
362_A 373_T 1.234 0.74
20_T 136_A 1.229 0.74
278_I 294_G 1.226 0.74
7_R 221_E 1.225 0.74
376_F 446_A 1.223 0.73
35_M 129_V 1.219 0.73
6_V 17_T 1.214 0.73
299_A 302_K 1.213 0.73
254_L 297_L 1.213 0.73
220_N 224_K 1.212 0.72
195_L 500_D 1.211 0.72
580_T 585_T 1.209 0.72
251_E 255_S 1.207 0.72
237_P 240_E 1.206 0.72
328_D 342_L 1.205 0.72
142_G 231_W 1.203 0.72
654_I 662_V 1.201 0.71
571_K 575_V 1.198 0.71
248_A 252_A 1.197 0.71
9_F 16_V 1.189 0.70
599_A 604_K 1.188 0.70
84_P 395_S 1.188 0.70
260_I 269_Q 1.182 0.70
536_V 540_A 1.18 0.70
632_I 662_V 1.174 0.69
358_T 598_A 1.17 0.69
243_N 247_A 1.169 0.68
421_F 456_P 1.168 0.68
378_F 442_S 1.165 0.68
324_I 464_I 1.161 0.68
357_G 361_D 1.158 0.67
185_A 513_G 1.157 0.67
139_S 219_I 1.151 0.67
199_D 520_Q 1.15 0.67
115_A 133_G 1.148 0.66
242_L 296_I 1.146 0.66
199_D 524_N 1.146 0.66
341_A 448_L 1.136 0.65
641_I 676_V 1.134 0.65
18_L 31_V 1.132 0.65
336_R 549_S 1.129 0.65
255_S 259_R 1.128 0.64
619_I 641_I 1.127 0.64
68_Y 428_V 1.121 0.64
381_N 428_V 1.114 0.63
17_T 34_S 1.113 0.63
375_T 418_M 1.108 0.62
599_A 605_A 1.106 0.62
119_S 337_T 1.106 0.62
201_M 502_I 1.105 0.62
374_D 405_R 1.101 0.62
364_V 373_T 1.097 0.61
48_K 271_D 1.096 0.61
333_V 342_L 1.094 0.61
157_I 172_E 1.093 0.61
309_V 469_V 1.093 0.61
352_V 378_F 1.087 0.60
19_E 34_S 1.086 0.60
556_H 597_I 1.086 0.60
57_F 109_N 1.084 0.60
29_A 44_V 1.081 0.60
443_V 496_A 1.08 0.59
256_D 259_R 1.079 0.59
350_A 441_A 1.077 0.59
20_T 31_V 1.075 0.59
181_G 212_Q 1.074 0.59
54_Q 57_F 1.069 0.58
382_F 386_S 1.064 0.58
479_S 532_H 1.064 0.58
611_R 614_E 1.063 0.58
7_R 222_L 1.062 0.58
414_V 461_V 1.061 0.57
197_S 200_Q 1.06 0.57
653_Q 690_K 1.059 0.57
269_Q 273_I 1.059 0.57
659_V 666_L 1.057 0.57
222_L 228_K 1.056 0.57
64_Q 88_E 1.055 0.57
44_V 133_G 1.051 0.56
411_V 450_L 1.047 0.56
267_Y 271_D 1.047 0.56
577_R 581_E 1.047 0.56
466_M 477_V 1.046 0.56
72_R 79_R 1.045 0.56
407_A 443_V 1.044 0.56
208_G 211_Q 1.044 0.56
54_Q 108_V 1.042 0.55
142_G 230_R 1.041 0.55
385_Y 432_T 1.041 0.55
44_V 143_I 1.04 0.55
189_V 507_M 1.04 0.55
559_K 592_D 1.038 0.55
246_V 277_T 1.038 0.55
632_I 668_M 1.037 0.55
659_V 664_D 1.037 0.55
23_M 39_A 1.032 0.54
331_T 541_I 1.031 0.54
619_I 642_G 1.028 0.54
159_D 174_K 1.026 0.54
10_Q 13_Q 1.024 0.53
567_D 575_V 1.022 0.53
414_V 459_A 1.021 0.53
433_E 438_S 1.019 0.53
212_Q 216_I 1.017 0.53
162_V 169_E 1.015 0.52
556_H 606_K 1.015 0.52
509_I 518_I 1.012 0.52
351_L 432_T 1.003 0.51
564_K 618_E 1.003 0.51
631_R 686_R 1.001 0.51
274_K 297_L 1 0.51
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3cdiA111000.306Contact Map0.641
4aidA30.99721000.322Contact Map0.638
1e3pA10.99161000.323Contact Map0.585
3u1kA40.86081000.421Contact Map0.735
3gmeA10.77221000.444Contact Map0.638
2nn6A10.44731000.795Contact Map0.402
2nn6B10.3461000.838Contact Map0.415
2je6B10.33611000.839Contact Map0.68
2po1A10.3461000.841Contact Map0.635
2wnrB30.33191000.846Contact Map0.646
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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