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OPENSEQ.org
SYI - Isoleucine--tRNA ligase
UniProt: P00956 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10492
Length: 938 (919)
Sequences: 3403
Seq/Len: 3.70
SYI
Paralog alert: 0.50 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: SYI SYL SYV
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
371_L 386_L 5.601 1.00
82_K 87_Y 5.152 1.00
30_A 34_D 4.467 1.00
260_D 297_K 4.274 1.00
36_D 39_G 4.248 1.00
601_R 607_I 4.083 1.00
374_V 382_I 4.031 1.00
265_Q 291_T 3.917 1.00
188_C 409_H 3.902 1.00
125_K 128_E 3.744 1.00
121_E 124_A 3.592 1.00
275_K 295_T 3.525 1.00
33_T 142_I 3.382 1.00
757_E 761_R 3.361 1.00
191_C 409_H 3.325 1.00
253_I 306_F 3.32 1.00
358_G 362_T 3.274 1.00
479_H 482_T 3.168 1.00
105_K 108_Q 3.104 1.00
188_C 191_C 3.087 1.00
302_E 319_L 3.084 1.00
439_K 449_Q 3.044 1.00
262_A 275_K 2.989 1.00
175_N 579_A 2.967 1.00
135_D 138_R 2.931 1.00
237_L 331_A 2.905 1.00
598_G 643_E 2.9 1.00
346_G 351_L 2.868 1.00
29_L 769_F 2.845 1.00
700_L 791_W 2.838 1.00
85_S 700_L 2.808 1.00
171_K 174_G 2.774 1.00
305_R 314_D 2.762 1.00
619_K 642_D 2.742 1.00
260_D 275_K 2.665 1.00
370_T 386_L 2.665 1.00
253_I 331_A 2.655 1.00
554_H 577_S 2.617 1.00
432_A 436_K 2.595 1.00
659_A 774_V 2.564 1.00
480_K 525_V 2.552 1.00
385_A 389_E 2.477 1.00
191_C 406_C 2.462 1.00
557_D 587_R 2.44 1.00
322_H 345_I 2.428 1.00
692_A 758_A 2.402 1.00
690_G 805_N 2.369 1.00
262_A 278_V 2.352 1.00
902_P 919_E 2.349 1.00
204_D 402_S 2.33 1.00
171_K 175_N 2.326 1.00
698_D 714_R 2.319 1.00
688_A 755_I 2.312 1.00
49_K 87_Y 2.291 1.00
82_K 704_E 2.291 1.00
437_E 440_G 2.28 1.00
691_C 722_E 2.277 1.00
206_T 400_Q 2.265 1.00
259_F 325_L 2.24 1.00
322_H 334_T 2.233 1.00
598_G 641_S 2.233 1.00
77_I 630_A 2.224 1.00
386_L 390_K 2.223 1.00
252_A 318_I 2.201 1.00
31_R 776_G 2.197 1.00
204_D 400_Q 2.191 1.00
118_T 121_E 2.187 1.00
442_Q 588_Q 2.171 1.00
363_Y 367_T 2.145 1.00
80_K 768_S 2.142 1.00
763_M 771_A 2.131 1.00
254_S 334_T 2.131 1.00
670_D 673_K 2.123 1.00
734_R 748_C 2.118 1.00
188_C 406_C 2.117 1.00
697_E 701_K 2.116 1.00
206_T 398_K 2.115 1.00
365_P 372_D 2.107 1.00
40_I 784_K 2.101 1.00
190_D 409_H 2.089 1.00
406_C 409_H 2.087 1.00
686_R 800_D 2.077 1.00
161_T 543_S 2.049 1.00
302_E 349_Y 2.032 1.00
385_A 388_Q 2.026 1.00
237_L 317_A 2.025 1.00
433_Q 582_G 2.024 1.00
31_R 35_D 2.02 1.00
138_R 142_I 2.011 1.00
43_A 46_K 1.982 1.00
81_S 85_S 1.972 1.00
436_K 439_K 1.957 1.00
694_A 805_N 1.954 1.00
214_F 250_N 1.954 1.00
45_K 48_K 1.953 1.00
409_H 479_H 1.918 1.00
382_I 385_A 1.915 1.00
441_V 574_L 1.913 1.00
134_V 155_L 1.903 1.00
386_L 389_E 1.903 1.00
174_G 490_L 1.89 1.00
910_D 922_G 1.885 1.00
375_N 378_K 1.878 1.00
124_A 128_E 1.876 1.00
439_K 453_E 1.867 1.00
97_C 157_M 1.864 1.00
149_D 152_H 1.863 1.00
435_L 453_E 1.862 1.00
479_H 521_A 1.862 1.00
701_K 705_A 1.858 1.00
79_V 89_S 1.848 1.00
695_A 714_R 1.842 1.00
213_A 236_S 1.83 0.99
588_Q 706_Y 1.826 0.99
277_L 280_S 1.802 0.99
566_H 591_T 1.8 0.99
839_A 869_T 1.799 0.99
341_D 344_V 1.783 0.99
42_R 88_D 1.768 0.99
270_A 293_L 1.765 0.99
84_L 764_A 1.763 0.99
318_I 345_I 1.755 0.99
262_A 295_T 1.746 0.99
20_D 23_K 1.745 0.99
143_R 618_N 1.744 0.99
343_Y 347_Q 1.736 0.99
409_H 482_T 1.731 0.99
441_V 584_A 1.727 0.99
320_G 345_I 1.724 0.99
920_I 928_V 1.722 0.99
82_K 85_S 1.721 0.99
81_S 704_E 1.721 0.99
72_K 141_F 1.714 0.99
307_T 312_G 1.708 0.99
213_A 269_Q 1.697 0.99
191_C 411_T 1.691 0.99
239_I 253_I 1.691 0.99
310_F 386_L 1.68 0.99
263_L 294_G 1.668 0.99
281_V 285_I 1.666 0.99
406_C 411_T 1.665 0.99
688_A 723_M 1.65 0.99
260_D 295_T 1.643 0.99
167_R 501_E 1.642 0.99
188_C 479_H 1.637 0.99
109_E 125_K 1.637 0.99
388_Q 396_V 1.633 0.99
254_S 320_G 1.632 0.99
497_A 501_E 1.624 0.98
217_V 305_R 1.614 0.98
364_L 367_T 1.609 0.98
703_Y 766_I 1.603 0.98
264_V 278_V 1.601 0.98
167_R 497_A 1.6 0.98
40_I 43_A 1.596 0.98
546_D 581_K 1.588 0.98
172_I 579_A 1.585 0.98
39_G 151_S 1.583 0.98
865_T 868_A 1.583 0.98
53_L 79_V 1.576 0.98
279_E 283_Q 1.575 0.98
683_V 807_A 1.57 0.98
615_D 619_K 1.566 0.98
47_G 88_D 1.555 0.98
494_E 498_K 1.553 0.98
109_E 132_T 1.551 0.98
180_K 419_P 1.547 0.98
256_A 259_F 1.546 0.98
697_E 796_F 1.54 0.98
785_Y 788_T 1.53 0.98
297_K 300_E 1.528 0.98
433_Q 436_K 1.527 0.98
707_D 710_E 1.526 0.97
263_L 270_A 1.524 0.97
306_F 317_A 1.52 0.97
482_T 521_A 1.515 0.97
238_V 394_L 1.514 0.97
30_A 33_T 1.504 0.97
692_A 696_Q 1.487 0.97
696_Q 762_W 1.484 0.97
192_R 360_D 1.482 0.97
711_V 766_I 1.481 0.97
63_S 140_D 1.476 0.97
273_L 281_V 1.475 0.97
188_C 411_T 1.474 0.97
677_K 680_E 1.458 0.96
443_W 589_V 1.457 0.96
84_L 761_R 1.456 0.96
334_T 345_I 1.453 0.96
170_G 174_G 1.447 0.96
166_I 500_V 1.446 0.96
276_D 325_L 1.445 0.96
904_C 921_C 1.443 0.96
145_G 769_F 1.442 0.96
356_P 364_L 1.441 0.96
444_I 588_Q 1.44 0.96
217_V 314_D 1.439 0.96
558_M 588_Q 1.438 0.96
254_S 318_I 1.428 0.96
921_C 924_C 1.426 0.96
46_K 49_K 1.424 0.96
237_L 253_I 1.422 0.96
356_P 363_Y 1.42 0.96
190_D 411_T 1.419 0.96
167_R 171_K 1.417 0.96
438_I 456_V 1.41 0.95
63_S 136_G 1.403 0.95
616_V 625_L 1.399 0.95
760_V 774_V 1.395 0.95
85_S 704_E 1.394 0.95
912_G 918_A 1.392 0.95
251_R 352_E 1.383 0.95
690_G 694_A 1.382 0.95
172_I 576_I 1.378 0.95
446_D 449_Q 1.377 0.95
50_T 88_D 1.373 0.95
51_F 558_M 1.361 0.94
282_M 290_Y 1.36 0.94
761_R 789_G 1.355 0.94
91_Y 148_G 1.352 0.94
358_G 364_L 1.352 0.94
64_I 144_L 1.351 0.94
655_I 719_C 1.35 0.94
190_D 406_C 1.348 0.94
558_M 590_L 1.343 0.94
696_Q 758_A 1.341 0.94
255_I 317_A 1.34 0.94
898_G 907_Y 1.339 0.94
337_G 356_P 1.339 0.94
437_E 578_T 1.334 0.93
247_L 310_F 1.331 0.93
904_C 924_C 1.329 0.93
896_A 907_Y 1.327 0.93
426_D 432_A 1.324 0.93
208_P 398_K 1.322 0.93
45_K 83_G 1.318 0.93
215_Q 392_A 1.317 0.93
139_K 142_I 1.315 0.93
693_K 696_Q 1.314 0.93
72_K 146_V 1.312 0.93
435_L 457_A 1.311 0.93
381_D 385_A 1.307 0.92
503_D 507_A 1.307 0.92
700_L 704_E 1.304 0.92
188_C 482_T 1.302 0.92
131_A 157_M 1.3 0.92
320_G 334_T 1.296 0.92
191_C 479_H 1.295 0.92
66_I 595_T 1.293 0.92
168_A 580_M 1.292 0.92
255_I 331_A 1.291 0.92
473_P 526_K 1.286 0.92
363_Y 379_A 1.282 0.91
251_R 354_A 1.276 0.91
760_V 786_V 1.275 0.91
685_D 734_R 1.274 0.91
479_H 524_Y 1.267 0.91
499_R 507_A 1.266 0.91
410_K 606_S 1.266 0.91
26_P 143_R 1.262 0.91
382_I 386_L 1.259 0.90
424_S 429_G 1.257 0.90
284_R 397_E 1.257 0.90
764_A 787_F 1.257 0.90
165_I 531_L 1.256 0.90
443_W 452_I 1.256 0.90
615_D 618_N 1.254 0.90
627_L 712_V 1.253 0.90
443_W 566_H 1.252 0.90
31_R 772_D 1.25 0.90
25_E 622_A 1.249 0.90
170_G 493_M 1.249 0.90
194_A 463_C 1.248 0.90
210_I 247_L 1.245 0.90
35_D 784_K 1.243 0.90
547_V 552_A 1.24 0.90
66_I 610_T 1.239 0.90
369_P 372_D 1.239 0.90
182_A 419_P 1.239 0.90
161_T 540_T 1.239 0.90
456_V 571_M 1.239 0.90
607_I 610_T 1.238 0.90
371_L 383_V 1.238 0.90
129_Y 133_Q 1.238 0.90
51_F 706_Y 1.236 0.89
616_V 645_L 1.225 0.89
265_Q 270_A 1.224 0.89
74_L 560_L 1.221 0.89
658_T 724_G 1.219 0.89
255_I 302_E 1.218 0.88
263_L 296_V 1.217 0.88
533_V 536_D 1.215 0.88
55_D 72_K 1.213 0.88
371_L 389_E 1.21 0.88
172_I 423_V 1.209 0.88
703_Y 711_V 1.208 0.88
825_Q 829_D 1.207 0.88
202_Y 402_S 1.205 0.88
4_Y 7_T 1.204 0.88
449_Q 453_E 1.203 0.88
252_A 334_T 1.2 0.87
861_F 906_H 1.196 0.87
619_K 646_K 1.195 0.87
790_E 793_E 1.191 0.87
215_Q 307_T 1.19 0.87
751_A 755_I 1.187 0.87
453_E 457_A 1.186 0.87
306_F 315_V 1.185 0.87
64_I 68_H 1.183 0.86
188_C 521_A 1.182 0.86
481_D 523_Q 1.182 0.86
262_A 292_I 1.18 0.86
839_A 867_G 1.179 0.86
203_Y 410_K 1.178 0.86
650_D 653_R 1.176 0.86
217_V 307_T 1.171 0.86
775_W 789_G 1.17 0.86
374_V 378_K 1.17 0.86
800_D 804_M 1.167 0.85
113_P 125_K 1.165 0.85
374_V 379_A 1.165 0.85
823_I 832_V 1.164 0.85
696_Q 792_Y 1.162 0.85
849_S 853_T 1.161 0.85
263_L 272_I 1.161 0.85
216_A 317_A 1.157 0.85
574_L 585_P 1.157 0.85
652_Y 767_L 1.157 0.85
688_A 718_F 1.156 0.85
103_E 126_C 1.155 0.85
545_V 556_A 1.153 0.84
155_L 158_D 1.151 0.84
525_V 528_P 1.148 0.84
409_H 521_A 1.148 0.84
357_V 376_V 1.148 0.84
571_M 575_M 1.146 0.84
425_M 430_L 1.145 0.84
745_R 749_Q 1.145 0.84
186_H 415_F 1.136 0.83
423_V 464_I 1.135 0.83
216_A 304_L 1.134 0.83
24_R 27_G 1.132 0.83
803_A 806_D 1.125 0.82
371_L 382_I 1.119 0.82
436_K 440_G 1.119 0.82
812_L 862_V 1.119 0.82
92_V 544_V 1.119 0.82
265_Q 268_G 1.116 0.82
376_V 414_I 1.116 0.82
758_A 761_R 1.114 0.82
731_I 748_C 1.114 0.82
758_A 762_W 1.112 0.81
738_A 744_A 1.11 0.81
489_T 492_L 1.106 0.81
747_S 751_A 1.105 0.81
254_S 345_I 1.103 0.81
180_K 478_V 1.103 0.81
694_A 802_E 1.101 0.81
760_V 771_A 1.1 0.80
294_G 300_E 1.099 0.80
141_F 146_V 1.098 0.80
681_M 689_V 1.096 0.80
658_T 728_L 1.095 0.80
256_A 321_D 1.094 0.80
313_F 354_A 1.091 0.80
507_A 511_L 1.089 0.80
99_G 103_E 1.088 0.79
541_H 576_I 1.087 0.79
853_T 856_G 1.084 0.79
499_R 503_D 1.082 0.79
81_S 700_L 1.079 0.79
132_T 136_G 1.078 0.79
215_Q 236_S 1.077 0.79
212_V 387_L 1.075 0.78
201_E 407_W 1.073 0.78
49_K 82_K 1.069 0.78
426_D 429_G 1.066 0.78
37_L 147_L 1.066 0.78
28_M 776_G 1.065 0.77
381_D 396_V 1.065 0.77
28_M 773_E 1.064 0.77
450_A 454_S 1.063 0.77
194_A 361_G 1.061 0.77
479_H 484_E 1.06 0.77
640_V 645_L 1.06 0.77
355_N 364_L 1.059 0.77
649_A 653_R 1.059 0.77
107_E 126_C 1.058 0.77
456_V 574_L 1.058 0.77
334_T 346_G 1.055 0.76
538_G 576_I 1.053 0.76
66_I 616_V 1.053 0.76
275_K 292_I 1.052 0.76
239_I 331_A 1.052 0.76
800_D 803_A 1.051 0.76
45_K 88_D 1.046 0.76
669_F 753_Y 1.045 0.76
85_S 791_W 1.044 0.75
467_Q 529_D 1.042 0.75
756_A 778_L 1.041 0.75
511_L 515_E 1.04 0.75
84_L 787_F 1.038 0.75
154_Y 160_K 1.036 0.75
319_L 349_Y 1.036 0.75
107_E 122_F 1.034 0.74
337_G 353_T 1.034 0.74
495_E 498_K 1.033 0.74
258_D 321_D 1.031 0.74
669_F 750_T 1.03 0.74
173_I 178_L 1.03 0.74
266_I 271_V 1.03 0.74
783_E 789_G 1.029 0.74
916_E 929_A 1.029 0.74
27_G 30_A 1.028 0.74
595_T 638_M 1.028 0.74
668_G 749_Q 1.027 0.74
877_P 892_A 1.024 0.73
362_T 378_K 1.023 0.73
31_R 37_L 1.022 0.73
425_M 459_R 1.022 0.73
211_D 395_H 1.021 0.73
402_S 405_C 1.02 0.73
539_S 576_I 1.018 0.73
27_G 773_E 1.017 0.73
691_C 694_A 1.016 0.73
371_L 374_V 1.015 0.72
93_P 150_W 1.015 0.72
282_M 287_V 1.015 0.72
437_E 584_A 1.012 0.72
692_A 755_I 1.011 0.72
216_A 306_F 1.009 0.72
914_V 918_A 1.006 0.72
562_G 594_F 1.006 0.72
503_D 511_L 1.003 0.71
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1ffyA10.96591000.133Contact Map0.615
1gaxA20.86891000.334Contact Map0.728
1ileA10.84121000.354Contact Map0.724
1wz2A20.90621000.392Contact Map0.669
1wkbA10.77191000.436Contact Map0.685
2v0cA10.79741000.477Contact Map0.681
4arcA10.791000.488Contact Map0.667
3ziuA20.63221000.6Contact Map0.695
1rqgA10.68021000.69Contact Map0.65
2csxA20.51491000.694Contact Map0.722
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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