OPENSEQ.org - ILVH - ECOLI GREMLIN

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ILVH - Acetolactate synthase isozyme 3 small subunit

UniProt:	P00894	Sequence MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLSRMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVKRNTEIFRGQIIDVTPSLYTVQLAGTSGKLDAFLASIRDVAKIVEVARSGVVGLSRGDKIMR	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG10499
Length:	163 (161)
Sequences:	1188
Seq/Len:	7.38

ILVH
Paralog alert: 0.04 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: ILVH ILVN

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
111_Q	123_Q	3.209	1.00
35_V	44_S	3.165	1.00
105_T	122_V	2.697	1.00
11_N	42_T	2.668	1.00
89_V	135_L	2.357	1.00
87_M	131_L	2.288	1.00
134_F	138_I	2.285	1.00
140_D	143_K	2.07	1.00
109_R	130_K	2.028	1.00
91_I	138_I	1.994	1.00
148_A	155_L	1.895	1.00
124_L	134_F	1.883	1.00
102_K	112_I	1.84	1.00
136_A	139_R	1.726	1.00
11_N	44_S	1.724	1.00
43_L	71_L	1.715	1.00
109_R	133_A	1.697	1.00
99_D	103_R	1.641	1.00
103_R	106_E	1.6	1.00
86_I	150_S	1.58	1.00
33_L	44_S	1.571	1.00
107_I	115_V	1.57	1.00
34_T	153_V	1.54	1.00
31_E	51_V	1.525	0.99
63_Q	66_K	1.52	0.99
9_L	15_A	1.482	0.99
52_G	56_V	1.441	0.99
92_Q	145_V	1.439	0.99
16_L	33_L	1.417	0.99
10_E	71_L	1.404	0.99
50_T	60_I	1.381	0.99
7_V	32_S	1.357	0.99
15_A	46_M	1.334	0.98
108_F	133_A	1.318	0.98
133_A	157_R	1.311	0.98
25_Q	50_T	1.302	0.98
11_N	37_P	1.3	0.98
126_G	130_K	1.298	0.98
20_I	46_M	1.285	0.98
58_E	62_K	1.283	0.98
121_T	155_L	1.28	0.98
85_E	149_R	1.274	0.98
19_V	46_M	1.273	0.98
87_M	124_L	1.235	0.97
12_E	69_D	1.224	0.97
29_N	35_V	1.211	0.96
97_G	101_V	1.205	0.96
16_L	35_V	1.199	0.96
109_R	157_R	1.197	0.96
109_R	126_G	1.194	0.96
107_I	112_I	1.183	0.96
105_T	110_G	1.161	0.95
3_R	75_E	1.159	0.95
72_R	145_V	1.158	0.95
93_A	101_V	1.133	0.94
119_L	158_G	1.126	0.94
102_K	106_E	1.118	0.94
89_V	134_F	1.116	0.94
32_S	49_Q	1.113	0.94
118_S	159_D	1.097	0.93
11_N	40_D	1.093	0.93
125_A	130_K	1.084	0.92
11_N	38_T	1.077	0.92
19_V	64_L	1.074	0.92
10_E	69_D	1.059	0.91
65_H	72_R	1.054	0.91
114_D	157_R	1.036	0.90
94_S	97_G	1.035	0.90
128_S	132_D	1.034	0.90
84_R	109_R	1.033	0.90
17_S	57_L	1.031	0.90
79_G	129_G	1.019	0.89
21_G	26_R	1.016	0.89
5_L	71_L	1.015	0.89
130_K	157_R	1.01	0.88
82_V	121_T	1	0.88

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
2f1fA	2	0.9939	100	0.202	Contact Map	0.662
2pc6A	4	0.9939	100	0.204	Contact Map	0.702
2fgcA	1	0.9877	100	0.218	Contact Map	0.567
2lvwA	2	0.5521	99.4	0.76	Contact Map	0.415
1y7pA	3	0.9693	98.6	0.823	Contact Map	0.496
2ko1A	2	0.5153	98.4	0.834	Contact Map	0.537
1zpvA	3	0.5337	98.3	0.838	Contact Map	0.31
2f06A	2	0.8098	98.3	0.84	Contact Map	0.362
1u8sA	2	0.5767	98.2	0.842	Contact Map	0.408
3n0vA	4	0.9571	97.9	0.855	Contact Map	0.507

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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