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OPENSEQ.org
complex_btk
Genes: A B A+B
Length: 120 259 360
Sequences: 1325 80416 86
Seq/Len: 11.04 310.49 0.24
MirrorTree (Pazo et al. 2001) 0.09
Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.

[Show HHblits results] - Maybe useful in deciding what regions to trim.
Δgene Ratio of paralogs Joined
A B Seq/Len
1 0.06 0.11 0.03
2 0.06 0.14 0.04
5 0.07 0.17 0.09
10 0.08 0.21 0.16
20 0.08 0.25 0.23
100 0.09 0.32 0.44
∞ 0.12 0.34 0.46
Paired alignment generation
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
Sequence A E-value:
Iterations:
Sequence B E-value:
Iterations:
Δgene MSA
Figure 1: The effect of Δgene on ratio of paralogs in the genome for each gene, and the number of sequences in the resulting joined alignment. Figure 2: Distribution of Δgene across all genomes. Figure 3: Current parameters. You can use the form to adjust the parameters and resubmit the job!
GREMLIN results (Scaled_score > 1):
Figure 4: The darker and larger the blue dots, the higher strength in coevolution. 		Inter Residue pairs sorted by strength in coevolution signal:
i j Scaled Score Prob I_Prob
24_F 104_D 1.29 0.25 0.00
24_F 52_L 1.28 0.24 0.00
78_L 181_W 1.20 0.21 0.00
64_Y 114_S 1.19 0.20 0.00
39_V 229_I 1.17 0.20 0.00
81_Y 70_I 1.14 0.18 0.00
74_T 243_T 1.13 0.18 0.00
9_S 109_M 1.12 0.18 0.00
79_I 114_S 1.10 0.17 0.00
38_S 169_L 1.09 0.17 0.00
79_I 180_I 1.08 0.16 0.00
79_I 52_L 1.06 0.16 0.00
27_R 126_N 1.04 0.15 0.00
35_Y 73_I 1.04 0.15 0.00
79_I 13_F 1.03 0.15 0.00
22_G 241_R 1.02 0.14 0.00
25_I 113_E 1.01 0.14 0.00
37_V 141_G 1.00 0.14 0.00
6_M 2_L 0.98 0.13 0.00
80_N 8_L 0.97 0.13 0.00
79_I 111_Y 0.95 0.12 0.00
67_A 85_Y 0.95 0.12 0.00
81_Y 39_E 0.95 0.12 0.00
25_I 123_A 0.94 0.12 0.00
56_V 104_D 0.94 0.12 0.00
28_D 229_I 0.94 0.12 0.00
56_V 176_S 0.94 0.12 0.00
2_Y 179_D 0.94 0.12 0.00
27_R 141_G 0.94 0.12 0.00
48_Q 14_G 0.94 0.12 0.00
56_V 184_G 0.93 0.12 0.00
81_Y 56_K 0.92 0.12 0.00
27_R 139_D 0.92 0.12 0.00
35_Y 8_L 0.92 0.11 0.00
23_G 46_A 0.91 0.11 0.00
75_I 181_W 0.91 0.11 0.00
27_R 119_H 0.91 0.11 0.00
4_K 11_G 0.90 0.11 0.00
64_Y 187_M 0.89 0.11 0.00
89_L 104_D 0.89 0.11 0.00
38_S 73_I 0.89 0.11 0.00
93_L 184_G 0.88 0.10 0.00
27_R 241_R 0.87 0.10 0.00
32_A 246_I 0.87 0.10 0.00
25_I 174_F 0.87 0.10 0.00
39_V 120_R 0.86 0.10 0.00
72_F 121_D 0.86 0.10 0.00
33_G 229_I 0.86 0.10 0.00
74_T 33_K 0.86 0.10 0.00
28_D 131_D 0.86 0.10 0.00
62_S 47_K 0.85 0.10 0.00
53_H 241_R 0.85 0.10 0.00
64_Y 138_S 0.85 0.10 0.00
15_L 75_E 0.84 0.10 0.00
93_L 127_C 0.84 0.10 0.00
14_L 239_D 0.84 0.10 0.00
35_Y 219_P 0.83 0.10 0.00
2_Y 72_I 0.83 0.09 0.00
68_E 249_S 0.83 0.09 0.00
55_V 46_A 0.83 0.09 0.00
13_Q 190_I 0.82 0.09 0.00
24_F 19_G 0.82 0.09 0.00
11_A 129_V 0.82 0.09 0.00
22_G 126_N 0.82 0.09 0.00
74_T 78_A 0.82 0.09 0.00
29_S 121_D 0.82 0.09 0.00
64_Y 127_C 0.82 0.09 0.00
66_L 110_E 0.82 0.09 0.00
81_Y 173_K 0.81 0.09 0.00
64_Y 35_G 0.81 0.09 0.00
79_I 161_V 0.81 0.09 0.00
24_F 224_E 0.81 0.09 0.00
13_Q 83_L 0.81 0.09 0.00
77_E 105_V 0.80 0.09 0.00
8_R 117_F 0.80 0.09 0.00
4_K 179_D 0.80 0.09 0.00
Figure 5: The i (protein A) and j (protein B) are positions as given in the query sequences.

Scaled Score = raw_score/average(raw_scores)
Prob = P(contact | scaled_score, seq/len)
I_Prob = P(contact | scaled_score, seq/len, top_inter_score)
ID Seq/Len Name Options I_Prob Status
5761 0.94 SH2-KD(Linker) Δgene:(0, 0) A:(1E-20, 8) B:(1E-20, 8) msa: HHblits (2015_06) 0.02 Done - Shared
5755 0.24 complex_btk Δgene:(1, 20) A:(1E-20, 8) B:(1E-20, 8) msa: HHblits (2015_06) 0.00 Done - Shared
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